Detection and quantification of remote microglial activation in rodent models of focal ischaemia using the TSPO radioligand CLINDE.

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dc.contributor.authorArlicot, Nen_AU
dc.contributor.authorPetit, Een_AU
dc.contributor.authorKatsifis, Aen_AU
dc.contributor.authorToutain, Jen_AU
dc.contributor.authorDivoux, Den_AU
dc.contributor.authorBodard, Sen_AU
dc.contributor.authorRoussel, Sen_AU
dc.contributor.authorGuilloteau, Den_AU
dc.contributor.authorBernaudin, Men_AU
dc.contributor.authorChalon, Sen_AU
dc.date.accessioned2011-01-14T04:46:41Zen_AU
dc.date.available2011-01-14T04:46:41Zen_AU
dc.date.issued2010-12-01en_AU
dc.date.statistics2010-12-01en_AU
dc.description.abstractPurpose: Neuroinflammation is involved in stroke pathophysiology and might be imaged using radioligands targeting the 18 kDa translocator protein (TSPO). Methods: We studied microglial reaction in brain areas remote from the primary lesion site in two rodent models of focal cerebral ischaemia (permanent or transient) using [125I]-CLINDE, a promising TSPO single photon emission computed tomography radioligand. Results: In a mouse model of permanent middle cerebral artery occlusion (MCAO), ex vivo autoradiographic studies demonstrated, besides in the ischaemic territory, accumulation of [125I]-CLINDE in the ipsilateral thalamus with a binding that progressed up to 3 weeks after MCAO. [125I]- CLINDE binding markedly decreased in animals preinjected with either unlabelled CLINDE or PK11195, while no change was observed with flumazenil pre-treatment, demonstrating TSPO specificity. In rats subjected to transient MCAO, [125I]-CLINDE binding in the ipsilateral thalamus and substantia nigra pars reticulata (SNr) was significantly higher than that in contralateral tissue. Moreover, [125I]-CLINDE binding in the thalamus and SNr was quantitatively correlated to the ischaemic volume assessed by MRI in the cortex and striatum, respectively. Conclusion: Clinical consequences of secondary neuronal degeneration in stroke might be better treated thanks to the discrimination of neuronal processes using in vivo molecular imaging and potent TSPO radioligands like CLINDE to guide therapeutic interventions. © 2010, Springer.en_AU
dc.identifier.citationArlicot, N., Petit, E., Katsifis, A., Toutain, J., Divoux, D., Bodard, S., Roussel, S., Guilloteau, D., Bernaudin, M., & Chalon, S.(2010). Detection and quantification of remote microglial activation in rodent models of focal ischaemia using the TSPO radioligand CLINDE. European Journal of Nuclear Medicine and Molecular Imaging, 37(12), 2371-2380. doi:10.1007/s00259-010-1598-7en_AU
dc.identifier.govdoc3112en_AU
dc.identifier.issn1619-7070en_AU
dc.identifier.issue12en_AU
dc.identifier.journaltitleEuropean Journal of Nuclear Medicine and Molecular Imagingen_AU
dc.identifier.pagination2371-2380en_AU
dc.identifier.urihttp://dx.doi.org/10.1007/s00259-010-1598-7en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2916en_AU
dc.identifier.volume37en_AU
dc.language.isoenen_AU
dc.publisherSpringeren_AU
dc.subjectRadiologyen_AU
dc.subjectRodentsen_AU
dc.subjectSingle photon emission computed tomographyen_AU
dc.subjectClathratesen_AU
dc.subjectCerebral arteriesen_AU
dc.subjectDiagnostic techniquesen_AU
dc.titleDetection and quantification of remote microglial activation in rodent models of focal ischaemia using the TSPO radioligand CLINDE.en_AU
dc.typeJournal Articleen_AU
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