The application of synchrotron radiation induced X-ray emission in the measurement of zinc and lead in Wistar rat ameloblasts

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dc.contributor.authorArora, Men_AU
dc.contributor.authorKennedy, BJen_AU
dc.contributor.authorRyan, CGen_AU
dc.contributor.authorBoadle, RAen_AU
dc.contributor.authorWalker, DMen_AU
dc.contributor.authorHarland, CLen_AU
dc.contributor.authorLai, Ben_AU
dc.contributor.authorCai, ZHen_AU
dc.contributor.authorVogt, Sen_AU
dc.contributor.authorZoellner, Hen_AU
dc.contributor.authorChan, SWYen_AU
dc.date.accessioned2008-02-19T04:07:54Zen_AU
dc.date.accessioned2010-04-30T05:01:55Zen_AU
dc.date.available2008-02-19T04:07:54Zen_AU
dc.date.available2010-04-30T05:01:55Zen_AU
dc.date.issued2007-10en_AU
dc.date.statistics2007-10en_AU
dc.description.abstractThe development of analytical techniques for the measurement of trace elements in cellular compartments of developing teeth remains an important methodological issue in dental research. Recent advances in third generation synchrotron facilities have provided high brilliance X-ray sources that can be effectively used to study trace element distributions in small spatial regions with low detection limits. The present study describes for the first time the application of synchrotron radiation induced X-ray emission (SRIXE) in measuring the distribution of zinc and lead in the ameloblasts of developing Wistar rat teeth. Wistar rats were fed a standard rat diet, containing the normal dietary requirements of zinc, ad libitum and exposed to 100ppm of lead in drinking water. Resin embedded sections of first mandibular molars were analysed using a 13.3keV incident monochromatic X-ray beam focussed to a 0.2μm spot. Characteristic X-rays arising from the entire thickness of the sample were measured using an energy dispersive detector for quantitative analysis of elemental concentrations. The results showed that intranuclear concentrations of zinc were greater than levels in the cytoplasm. Furthermore, nuclear and cytoplasmic concentrations of zinc in the maturation stage (742±27 and 424±25ppm, respectively) were significantly higher than the zinc levels observed in the nucleus and cytoplasm of presecretory stage ameloblasts (132±10 and 109±10ppm, respectively) (p<0.05). A clear lead signal above the background was not detected in the ameloblasts and lead concentrations could only be reliably measured in the developing enamel. Overall, SRIXE was an effective method of studying the spatial distribution of zinc in the cells of developing teeth and offered a unique combination of sub-micron spatial resolution and parts-per-million detection limits (0.8–1 and 0.6–1ppm for zinc and lead, respectively). © 2007, Elsevier Ltd.en_AU
dc.identifier.citationArora, M., Kennedy, B. J., Ryan, C. G., Boadle, R. A., Walker, D. M., Harland, C. L., Lai, B., Cai, Z. H., Vogt, S., Zoellner, H., & Chan, S. W. Y. (2007). The application of synchrotron radiation induced X-ray emission in the measurement of zinc and lead in Wistar rat ameloblasts. Archives of Oral Biology, 52(10), 938-944. doi:10.1016/j.archoralbio.2007.04.003en_AU
dc.identifier.govdoc1115en_AU
dc.identifier.issn0003-9969en_AU
dc.identifier.issue10en_AU
dc.identifier.journaltitleArchives of Oral Biologyen_AU
dc.identifier.pagination938-944en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.archoralbio.2007.04.003en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/996en_AU
dc.identifier.volume52en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectRatsen_AU
dc.subjectX-ray emission analysisen_AU
dc.subjectSynchrotron radiationen_AU
dc.subjectElementsen_AU
dc.subjectTrace amountsen_AU
dc.subjectZincen_AU
dc.subjectLeaden_AU
dc.titleThe application of synchrotron radiation induced X-ray emission in the measurement of zinc and lead in Wistar rat ameloblastsen_AU
dc.typeJournal Articleen_AU
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