Charge transfer through DNA: a selective electrochemical DNA biosensor

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dc.contributor.authorWong, ELSen_AU
dc.contributor.authorGooding, JJen_AU
dc.date.accessioned2009-06-09T05:29:14Zen_AU
dc.date.accessioned2010-04-30T05:01:44Zen_AU
dc.date.available2009-06-09T05:29:14Zen_AU
dc.date.available2010-04-30T05:01:44Zen_AU
dc.date.issued2006-04-01en_AU
dc.date.statistics2006-04-01en_AU
dc.description.abstractThe charge-transfer properties of DNA duplexes are exploited to produce a fast, simple, sensitive, and selective DNA biosensor by exposing the DNA recognition interface to a sample containing target DNA and the redox-active intercalator, anthraquinonemonosulfonic acid (AQMS). Electrochemistry from electron transfer through the DNA to AQMS intercalated into DNA duplexes can be differentiated from electrochemistry due to direct access of the AQMS to the electrode surface due to the difference in the environment of the AQMS giving a shift in the potential at which the molecule is reduced. The ability to distinguish between the two electrochemical signals enables DNA hybridization to be monitored in real time. This in situ detection scheme has good selectivity, being able to differentiate between a complementary target DNA sequence and one containing either C-A or G-A singlebase mismatches. The concentration detection limit of the biosensor is 0.5 nM (1 pmol) with an assay time of 1 h. The fact that the end user is only required to simultaneously add the sample containing the target DNA and AQMS gives a DNA biosensor that is highly compatible with PCR on chip technologies. © 2006, American Chemical Societyen_AU
dc.identifier.citationWong, E. L. S., & Gooding, J. J. (2006). Charge transfer through DNA: a selective electrochemical DNA biosensor. Analytical Chemistry, 78(7), 2138-2144. doi:10.1021/ac0509096en_AU
dc.identifier.govdoc1101en_AU
dc.identifier.issn0003-2700en_AU
dc.identifier.issue7en_AU
dc.identifier.journaltitleAnalytical Chemistryen_AU
dc.identifier.pagination2138-2144en_AU
dc.identifier.urihttp://dx.doi.org/10.1021/ac0509096en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/1312en_AU
dc.identifier.volume78en_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Societyen_AU
dc.subjectDNAen_AU
dc.subjectElectron transferen_AU
dc.subjectElectrochemistryen_AU
dc.subjectHybridizationen_AU
dc.subjectDNA sequencersen_AU
dc.subjectChargesen_AU
dc.titleCharge transfer through DNA: a selective electrochemical DNA biosensoren_AU
dc.typeJournal Articleen_AU
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