Small-angle x-ray scattering reveals the N-terminal domain organization of cardiac myosin binding protein C

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dc.contributor.authorJeffries, CMen_AU
dc.contributor.authorWhitten, AEen_AU
dc.contributor.authorHarris, SPen_AU
dc.contributor.authorTrewhella, Jen_AU
dc.date.accessioned2009-06-16T04:17:07Zen_AU
dc.date.accessioned2010-04-30T05:01:36Zen_AU
dc.date.available2009-06-16T04:17:07Zen_AU
dc.date.available2010-04-30T05:01:36Zen_AU
dc.date.issued2008-04-04en_AU
dc.date.statistics2008-04-04en_AU
dc.description.abstractMyosin binding protein C (MyBP-C) is a multidomain accessory protein of striated muscle sarcomeres. Three domains at the N-terminus of MyBP-C (Cl-m-C2) play a crucial role in maintaining and modulating actomyosin interactions. The cardiac isoform has an additional N-terminal domain (CO) that is postulated to provide a greater level of regulatory control in cardiac muscle. We have used small-angle X-ray scattering, ab initio shape restoration, and rigid-body modeling to determine the average shape and spatial arrangement of the four N-terminal domains of cardiac MyBP-C (C0C2) and a three-domain variant that is analogous to the N-terminus of the skeletal isoform (C1C2). We found that the domains of both proteins are tandemly arranged in a highly extended configuration that is sufficiently long to span the interfilament cross-bridge distances in vivo and, hence, be poised to modulate these interactions. The average spatial organization of the C1, m, and C2 domains is not significantly perturbed by the removal of the cardiac-specific CO domain, suggesting that the interdomain interfaces, while relatively small in area, have a degree of rigidity. Modeling the C0C2 and C1C2 scattering data reveals that the structures of the C0 and m domains (also referred to as the 'MyBP motif') are compact and have dimensions that are consistent with the immunoglobulin fold superfamily of proteins. Sequence analysis, homology modeling, and circular dichroism experiments support the conclusion that the previously undetermined structures of these domains can be characterized as having an immunoglobulin-like fold. Atomic models using the known NMR structures for C1 and C2 as well as homology models for the C0 and m domains provide insights into the placement of conserved serine residues of the m domain that are phosphorylated in vivo and cause a change in muscle fiber contraction by abolishing interactions with myosin. © 2008, Elsevier Ltd.en_AU
dc.identifier.citationJeffries, C. M., Whitten, A. E., Harris, S. P., & Trewhella, J. (2008). Small-angle x-ray scattering reveals the N-terminal domain organization of cardiac myosin binding protein C. Journal of Molecular Biology, 377(4), 1186-1199. doi:10.1016/j.jmb.2008.01.080en_AU
dc.identifier.govdoc1093en_AU
dc.identifier.issn0022-2836en_AU
dc.identifier.issue4en_AU
dc.identifier.journaltitleJournal of Molecular Biologyen_AU
dc.identifier.pagination1186-1199en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.jmb.2008.01.080en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/1375en_AU
dc.identifier.volume377en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectBinding energyen_AU
dc.subjectImmunoglobulinsen_AU
dc.subjectMyosinen_AU
dc.subjectProteinsen_AU
dc.subjectMusclesen_AU
dc.titleSmall-angle x-ray scattering reveals the N-terminal domain organization of cardiac myosin binding protein Cen_AU
dc.typeJournal Articleen_AU
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