Nuclear microprobe studies of metal(loid)s distribution in hyperaccumulating plants

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dc.contributor.authorKachenko, AGen_AU
dc.contributor.authorSingh, Ben_AU
dc.contributor.authorSiegele, Ren_AU
dc.contributor.authorBhatia, NPen_AU
dc.date.accessioned2009-12-03T23:27:59Zen_AU
dc.date.accessioned2010-04-30T04:56:41Zen_AU
dc.date.available2009-12-03T23:27:59Zen_AU
dc.date.available2010-04-30T04:56:41Zen_AU
dc.date.issued2007-11en_AU
dc.date.statistics2007-11en_AU
dc.description.abstractMicro-proton-induced X-ray emission (μ-PIXE) spectroscopy was used to determine in situ elemental concentrations of nickel (Ni) and arsenic (As) in leaf and stem tissues of hyperaccumulating plants Hybanthus floribundus subsp floribundus and Pityrogramma calomelanos var. austroamericana, respectively. Nickel concentration in seeds of H. floribundus subsp floribundus was also investigated. Both species were grown in metal(loid) contaminated potting mix for 20 weeks duration under controlled glasshouse conditions. Leaf and stem samples were hand-sectioned, cryo-fixed and freeze-dried in liquid nitrogen before μ-PIXE analysis using the 10-MV tandem accelerator at the Australian Nuclear Science and Technology Organization. In H. floribundus subsp floribundus leaves, Ni was highest in the adaxial epidermal cells (1% dry weight; DW) and least in spongy mesophyll (0.53% DW). In stem tissues, Ni concentrations were highest in the collenchyma (0.25% DW) and there was no clear pattern of Ni localization in seeds. In P. calomelanos pinnules, As localization was relatively uniform across the whole specimen and in stipe tissues, highest concentration occurred in the vascular bundle (0.2% DW). These results suggest that hyperaccumulating plants sequester excess metal(loid)s in different cellular loci and enables us to better understand the physiology and ecology of these hyperaccumulating species.en_AU
dc.description.sponsorshipAustralian Institute of Nuclear Science and Engineering (AINSE); Vacuum Society of Australia (VSA); Australian Research Council (ARC); Australian Research Network for Advanced Materials (ARNAM); JAVAC; Nanotechnology Network; ThermoFisher Scientificen_AU
dc.identifier.citationKachenko, A. G., Singh, B., Siegele, R., & Bhatia, N. (2007). Nuclear microprobe studies of metal(loid)s distribution in hyperaccumulating plants. Poster presented to the 15th Australian Conference on Nuclear and Complementary Techniques of Analysis and 9th Vacuum Society of Australia Congress, 21st – 23rd November 2007. Melbourne, Australia: University of Melbourne.en_AU
dc.identifier.conferenceenddate23 November 2007en_AU
dc.identifier.conferencename15th Australian Conference on Nuclear and Complementary Techniques of Analysis and 9th Vacuum Society of Australia Congressen_AU
dc.identifier.conferenceplaceMelbourne, Australiaen_AU
dc.identifier.conferencestartdate21 November 2007en_AU
dc.identifier.govdoc1017en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2571en_AU
dc.language.isoenen_AU
dc.publisherAustralian Institute of Nuclear Science and Engineering (AINSE)en_AU
dc.subjectSemimetalsen_AU
dc.subjectPlantsen_AU
dc.subjectPIXE analysisen_AU
dc.subjectNickelen_AU
dc.subjectArsenicen_AU
dc.subjectAcceleratorsen_AU
dc.titleNuclear microprobe studies of metal(loid)s distribution in hyperaccumulating plantsen_AU
dc.typeConference Posteren_AU
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