Sugar distribution between and around membranes during dehydration

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dc.contributor.authorBryant, Gen_AU
dc.contributor.authorLenné, Ten_AU
dc.contributor.authorKoster, KLen_AU
dc.contributor.authorGarvey, CJen_AU
dc.date.accessioned2009-12-08T03:31:40Zen_AU
dc.date.accessioned2010-04-30T04:56:30Zen_AU
dc.date.available2009-12-08T03:31:40Zen_AU
dc.date.available2010-04-30T04:56:30Zen_AU
dc.date.issued2006-12en_AU
dc.date.statistics2006-12en_AU
dc.description.abstractIt is now well known that small solutes such as sugars can reduce membrane damage during freezing and dehydration. One of the mechanisms for this protection is that small solutes can hinder deleterious phase transitions, thus preserving membrane integrity. The mechanisms for this can be understood in terms of the hydration forces explanation (HFE) [G. Bryant, K.L. Koster, J. Wolfe, Seed Sci. Res. 11 (2001) 17–25]. At low to intermediate hydrations, the presence of small solutes reduces membrane transition temperatures through their (non-specific) volumetric and osmotic properties. If concentrations are sufficiently high that vitrification occurs, then the transition temperatures are depressed even further. In the fully dehydrated state, direct hydrogen bonding between solutes and membranes may become important. Recently [K. Koster, G. Bryant, Eur. Biophys. J. 32 (2003) 96–105], we showed how solutes can be excluded from inter-membrane regions during dehydration if the solutes are too large, explaining why large solutes (eg polymers) cannot inhibit membrane phase transition. This raises two questions: (1) is there partial exclusion of small and intermediate sized solutes? (2) if so, how does this alter the effects of the solutes? and (3) is there a maximum useful concentration of small solutes? Question (3) is answered in an accompanying paper (see Lenné et al., this volume). In this paper we will present the results of Small Angle Neutron Scattering (SANS) and Small angle X-ray scattering (SAXS) experiments designed to answer the first two questions, and discuss the implications for our understanding of the effects of solutes on membranes during dehydration. © 2010, Elsevier Ltd.en_AU
dc.description.sponsorshipSociety for Cryobiology; Society for Low Temperature Biologyen_AU
dc.identifier.citationBryant, G., Lenné, T., Koster, K. L., & Garvey, C. J. (2006). Sugar distribution between and around membranes during dehydration. 43rd Annual Meeting of the Society for Cryobiology in association with the Society for Low Temperature Biology (Cryo 2006), 24th – 27th July 2006. Hamburg, Germany: Chamber of Commerce. In Cryobiology, 53(3), 404. doi:10.1016/j.cryobiol.2006.10.086en_AU
dc.identifier.conferenceenddate27 July 2006en_AU
dc.identifier.conferencename43rd Annual Meeting of the Society for Cryobiology in association with the Society for Low Temperature Biology (Cryo 2006)en_AU
dc.identifier.conferenceplaceHamburg, Germanyen_AU
dc.identifier.conferencestartdate24 July 2006en_AU
dc.identifier.govdoc1008en_AU
dc.identifier.issn0011-2240en_AU
dc.identifier.issue3en_AU
dc.identifier.journaltitleCryobiologyen_AU
dc.identifier.pagination404en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.cryobiol.2006.10.086en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/2649en_AU
dc.identifier.volume53en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectSaccharidesen_AU
dc.subjectFreezingen_AU
dc.subjectDehydrationen_AU
dc.subjectMembranesen_AU
dc.subjectDistribution functionsen_AU
dc.subjectSmall angle scatteringen_AU
dc.titleSugar distribution between and around membranes during dehydrationen_AU
dc.typeConference Presentationen_AU
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