Preliminary SAXS and SANS studies of protein-based adhesive elastomer secreted by the Australian frog Notaden bennetti

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dc.contributor.authorGraham, LDen_AU
dc.contributor.authorWhite, JWen_AU
dc.contributor.authorKnott, RBen_AU
dc.date.accessioned2022-05-26T05:02:07Zen_AU
dc.date.available2022-05-26T05:02:07Zen_AU
dc.date.issued2005-11-27en_AU
dc.date.statistics2022-05-26en_AU
dc.descriptionPhysical copy held by ANSTO Library at DDC 539.7217/2en_AU
dc.description.abstractWhen provoked, Notaden bennetti frogs secrete an exudate which rapidly forms a tacky elastic solid (frog glue). This protein-based material acts as a promiscuous pressure-sensitive adhesive that functions even in wet conditions. The adhesive strength and elasticity if the glue make this an attractive material for orthopedic repairs and other surgical procedures, possibly including topical would closure. Protein analysis of the denaturing gel electrophoresis revels a characteristic pattern of bands with apparent molecular masses of 13-500 kDa, with the largest protein also being the most abundant one. This large protein (called Nb-1R) appears to be the major structural component of the glue. Key issues understanding of the function of all proteins are shape and 3D structure. The Small Angle X-Ray and Neutron Scattering (SAXS and SANS) techniques are well suited to explore the shape of proteins in solution and these techniques have been applied to various forms of the frog glue, Scattering results indicated an Rg of 2.0-3,6nm, which is consistent with globular proteins of 30-170kDa. One possible explanation to the difference between the molecular mass ranges deduced from gel electrophoresis and scattering data is the dominant protein is elongated rather than spherical.en_AU
dc.identifier.booktitleFinal Programme and Abstract Booken_AU
dc.identifier.citationGraham, L. D., White, J. W., & Knott, R. B. (2005). Preliminary SAXS and SANS studies of protein-based adhesive elastomer secreted by the Australian frog Notaden bennetti. Paper presented at the Eighth International Conference on Neutron Scattering ICNS 2005: "Neutrons for structure and dynamics - a new era", Sydney Convention & Exhibition Centre, Sydney, Australia, 27 November-2 December 2005. In Final Programme and Abstract Book, (pp. 281).en_AU
dc.identifier.conferenceenddate2 December 2005en_AU
dc.identifier.conferencenameEighth International Conference on Neutron Scattering ICNS 2005: 'Neutrons for structure and dynamics - a new eraen_AU
dc.identifier.conferenceplaceSydney, Australiaen_AU
dc.identifier.conferencestartdate27 November 2005en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/13214en_AU
dc.language.isoenen_AU
dc.publisherThe Bragg Institute, Australian Nuclear Science and Technology Organisationen_AU
dc.subjectFrogsen_AU
dc.subjectProteinsen_AU
dc.subjectAdhesivesen_AU
dc.subjectElasticityen_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectSurgical materialsen_AU
dc.subjectElectrophoresisen_AU
dc.subjectGelsen_AU
dc.subjectAustraliaen_AU
dc.titlePreliminary SAXS and SANS studies of protein-based adhesive elastomer secreted by the Australian frog Notaden bennettien_AU
dc.typeConference Abstracten_AU
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