Synchrotron macro ATR-FTIR: where we are and what’s next for live-cell measurement

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dc.contributor.authorVongsvivut, JPen_AU
dc.contributor.authorPérez-Guaita, Den_AU
dc.contributor.authorNankervis, Len_AU
dc.contributor.authorMassey, Aen_AU
dc.contributor.authorAmpt, Cen_AU
dc.contributor.authorMcKinlay, Jen_AU
dc.contributor.authorSandt, Cen_AU
dc.contributor.authorTobin, MJen_AU
dc.date.accessioned2024-01-12T01:04:29Zen_AU
dc.date.available2024-01-12T01:04:29Zen_AU
dc.date.issued2020-11-19en_AU
dc.date.statistics2023-12-11en_AU
dc.description.abstractThis presentation aims to provide a summary on the recent applications of our synchrotron macro ATR-FTIR microspectroscopy, unique to the Australian Synchrotron’s Infrared Microspectroscopy (IRM) beamline. The technique provides molecular information with sub-cellular resolution down to 1-2 m beyond the resolution limit allowed for standard synchrotron-FTIR setups and further simplifies otherwise complicated sample preparation [1]. Since the technique was made available for users in 2016, this high-resolution chemical mapping capability has facilitated diverse experiments on the beamline expanding its applications into many new areas. Some of the recent examples include novel environmental sustainable geopolymer concretes [2,3], archaeological bones [4] and spider silk cross-sections [5]. The second part of the presentation will highlight further development of the macro ATR-FTIR technique specifically for live-cell measurement in an aqueous environment. Through the collaboration with the SMIS beamline at SOLEIL (France), we undertook a beamtime experiment using their inverted ATR-FTIR accessory to acquire spectra from live red blood cells. The experience and knowledge gained from this international beamtime experiment, together with the effort from our mechanical engineering team, have resulted in an optical design to be developed into the first prototype of ATR-FTIR setup for live-cell measurement. References [1] J. Vongsvivut, D. Pérez-Guaita, B. R. Wood, P. Heraud, K. Khambatta, D. Hartnell, M. J. Hackett, and M. J. Tobin, “Synchrotron Macro ATR-FTIR Microspectroscopy for High-Resolution Chemical Mapping of Single Cells,” Analyst 144, 10, 3226-3238 (2019). [2] A. Hajimohammadi, T. Ngo, J. L. Provis, T. Kim, and J. Vongsvivut, “High Strength/Density Ratio in a Syntactic Foam Made from One-Part Mix Geopolymer and Cenospheres,” Composites Part B, 173, 106908 (2019). [3] A. Hajimohammadi, T. Ngo, and J. Vongsvivut, “Interfacial Chemistry of a Fly Ash Geopolymer and Aggregates,” Journal of Cleaner Production, 231, 980-989 (2019). [4] J. J. Miszkiewicz, C. Rider, S. Kealy, C. Vrahnas, N. A. Sims, J. Vongsvivut, M. J. Tobin, M. J. L. A. Bolunia, A. S. De Leon, A. L. Peñalosa, P. S. Pagulayan, A. V. Soriano, R. Page, and M. F. Oxenham, “Asymmetric Midshaft Femur Remodelling in an Adult Male with Left Sided Hip Joint Ankylosis, Metal Period Nagsabaran, Philippines,” International Journal of Palaeopathology, 31, 14 (2020). [5] C. Haynl, J. Vongsvivut, K. R. H. Mayer, H. Bargel, V. J. Neubauer, M. J. Tobin, M. A. Elgar, and T. Scheibel, “Dimensional Stability of a Remarkable Spider Foraging Web Achieved by Synergistic Arrangement of Silk Fibers,” accepted for publication in Scientific Reports (2020)en_AU
dc.description.sponsorshipWe would like to acknowledge the financial support from International Synchrotron Access Program (ISAP No. AS/IA182/14167) to perform the live-cell ATR-FTIR experiment at SOLEIL’s SMIS beamline (Proposal ID. 20180157).en_AU
dc.identifier.articlenumber204en_AU
dc.identifier.booktitleAustralian Synchrotron User Meeting 2020, 19 November 2020 - 20 November 2020: Book of Abstractsen_AU
dc.identifier.citationVongsvivut, J. P., David Pérez-Guaita, D., Nankervis, L., Massey, A., Ampt, C., McKinlay, J.,Sandt, C., & Tobin, M. (2020). Synchrotron macro ATR-FTIR: where we are and what’s next for live-cell measurement. Abstract presented at the Australian Synchrotron User Meeting 2020, Melbourne, Victoria, 19 November 2020 - 20 November 2020. In Australian Synchrotron User Meeting 2020, 19 November 2020 - 20 November 2020: Book of Abstracts, 204, pp. 59. Retrieved from: https://events01.synchrotron.org.au/event/122/book-of-abstracts.pdf#page=66en_AU
dc.identifier.conferenceenddate2020-11-20en_AU
dc.identifier.conferencenameAustralian Synchrotron User Meeting 2020en_AU
dc.identifier.conferenceplaceMelbourne, Victoriaen_AU
dc.identifier.conferencestartdate2020-11-19en_AU
dc.identifier.pagination59en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15340en_AU
dc.language.isoenen_AU
dc.publisherAustralian Nuclear Science and Technology Organisationen_AU
dc.relation.urihttps://events01.synchrotron.org.au/event/122/book-of-abstracts.pdf#page=66en_AU
dc.subjectAnimal cellsen_AU
dc.subjectSpidersen_AU
dc.subjectSynchrotronsen_AU
dc.subjectSpectroscopyen_AU
dc.subjectResolutionen_AU
dc.subjectPolymersen_AU
dc.subjectConcretesen_AU
dc.subjectSkeletonen_AU
dc.titleSynchrotron macro ATR-FTIR: where we are and what’s next for live-cell measurementen_AU
dc.typeConference Abstracten_AU
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