Quantification of dopamine d2 receptor density and apparent affinity can be used to longitudinally assess transient striatal variations during adolescence using [11c]raclopride pet imaging

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dc.contributor.authorCallaghan, PDen_AU
dc.contributor.authorSobbi, PFen_AU
dc.contributor.authorSafavi-Naeini, Men_AU
dc.contributor.authorWimberley, CAen_AU
dc.contributor.authorDavis, Een_AU
dc.contributor.authorZahra, Den_AU
dc.contributor.authorArthur, Aen_AU
dc.contributor.authorRahardjo, GLen_AU
dc.contributor.authorPerkins, Gen_AU
dc.contributor.authorPascali, Gen_AU
dc.contributor.authorReilhac-Laborde, Aen_AU
dc.contributor.authorGrégoire, MCen_AU
dc.date.accessioned2023-02-01T23:24:22Zen_AU
dc.date.available2023-02-01T23:24:22Zen_AU
dc.date.issued2017-04-11en_AU
dc.date.statistics2022-09-18en_AU
dc.description.abstractBackground Transient increases in striatal dopamine D2 receptors occur during adolescence in rats, correlating with a developmental epoch where synaptic pruning occurs. Alteration of these processes with external stresses during adolescence may lead to affective disorders later in life. Longitudinal PET imaging with [11C]raclopride using a partial saturation design allows assessment of density (Bavail) and affinity changes (appKd) to map neurodevelopmental changes in D2 expression, which necessitates a significant level of receptors occupancy during the PET study. Aims Validate that repeated transient partial saturation of D2 receptors does not bias measures of D2 Bavail and appKd assessed using PET/CT imaging with [11C]raclopride. Methods Three cohorts of male Sprague-Dawley rats (n=6-7/group) underwent a single session of PET/CT imaging (INVEON, Siemens, USA) with [11C]raclopride (5 nmol injected i.v.) as naïve or after repeated partial saturation of D2 receptors: Cohort A received 5nmol raclopride (i.v) weekly from PND35 (postnatal day) to PND96 with PET imaging session at PND96, cohort B was scanned at PND96; Cohort C was scanned at PND35 Datasets were reconstructed (2D-FBP), coregistered with CT and time-activity data extracted using age matched atlas-based volumes of interest (striatum, cerebellum). in vivo receptor density and appKd were derived using kinetic modelling (comparisons used 1-way ANOVA follow by post hoc test). Results Expected differences in Bavail and appKd were seen between the adolescent (PND35) and the adult (PND96) cohorts, corresponding with increases in D2 receptor consistently reported in the literature using post mortem methods. No significant difference was observed in both Bavail and appKd in cohort A, exposed to repeated D2 partial saturation, compared to the naïve cohort B. Conclusion Longitudinal quantification of dopamine D2 receptor density and apparent affinity in vivo using [11C]raclopride PET imaging with partial saturation can be used to map changes in adolescent and adult rats.en_AU
dc.identifier.citationCallaghan, P., Sobbi, P. F., Safavi-Naeini, M., Wimberley, C., Davis, E., Zahra, D., Arthur, A., Rahardjo, G., Perkins, G., Pascali, G., Reilhac-Laborde, A., & Gregoire, M. C. (2017). Quantification of dopamine d2 receptor density and apparent affinity can be used to longitudinally assess transient striatal variations during adolescence using [11c]raclopride pet imaging. Presentation to the 47th Annual Scientific Meeting of the Australian and New Zealand Society of Nuclear Medicine Hobart, Tasmania, 21–23 Apr 2017. In Internal Medicine Journal, 47(S1), 5-23. doi:10.1111/imj.13422en_AU
dc.identifier.conferenceenddate23 April 2017en_AU
dc.identifier.conferencename47th Annual Scientific Meeting of the Australian and New Zealand Society of Nuclear Medicine Hobarten_AU
dc.identifier.conferenceplaceTasmania, Australiaen_AU
dc.identifier.conferencestartdate21 April 2017en_AU
dc.identifier.issn1444-0903en_AU
dc.identifier.issueS1en_AU
dc.identifier.journaltitleInternal Medicine Journalen_AU
dc.identifier.pagination45047en_AU
dc.identifier.urihttps://doi.org/https://doi.org/10.1111/imj.13422en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14593en_AU
dc.identifier.volume47en_AU
dc.language.isoenen_AU
dc.publisherJohn Wiley & Sons, Inc.en_AU
dc.subjectDopamineen_AU
dc.subjectReceptorsen_AU
dc.subjectAdolescentsen_AU
dc.subjectPositron computed tomographyen_AU
dc.subjectRatsen_AU
dc.subjectCAT scanningen_AU
dc.subjectInjectionen_AU
dc.subjectDensityen_AU
dc.subjectDataen_AU
dc.titleQuantification of dopamine d2 receptor density and apparent affinity can be used to longitudinally assess transient striatal variations during adolescence using [11c]raclopride pet imagingen_AU
dc.typeConference Presentationen_AU
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