Biochemical interaction of few layer black phosphorus with microbial cells using synchrotron macro-ATR-FTIR

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dc.contributor.authorShaw, ZLen_AU
dc.contributor.authorCheeseman, Sen_AU
dc.contributor.authorHuang, LZYen_AU
dc.contributor.authorPenman, Ren_AU
dc.contributor.authorAhmed, Ten_AU
dc.contributor.authorBryant, SJen_AU
dc.contributor.authorBryant, Gen_AU
dc.contributor.authorChristofferson, AJen_AU
dc.contributor.authorOrwell-Twigg, Ren_AU
dc.contributor.authorDekiwadia, Cen_AU
dc.contributor.authorTruong, VKen_AU
dc.contributor.authorVongsvivut, JPen_AU
dc.contributor.authorWalia, Sen_AU
dc.contributor.authorElbourne, Aen_AU
dc.date.accessioned2023-02-01T23:23:32Zen_AU
dc.date.available2023-02-01T23:23:32Zen_AU
dc.date.issued2022-06-01en_AU
dc.date.statistics2022-07-22en_AU
dc.description.abstractIn the fight against drug-resistant pathogenic microbial cells, low dimensional materials are emerging as a promising alternative treatment. Specifically, few-layer black phosphorus (BP) has demonstrated its effectiveness against a wide range of pathogenic microbial cells with studies suggesting low cytotoxicity towards healthy mammalian cells. However, the antimicrobial mechanism of action of BP is not well understood and further in-depth investigations are required. In this work, the complex biochemical interaction between BP and a series of microbial cells is investigated using advanced, high-resolution microscopy techniques to provide a greater understanding of the antimicrobial mechanism. Synchrotron macro-attenuated total reflection–Fourier transform infrared (ATR-FTIR) micro-spectroscopy is used to elucidate the chemical changes occurring outside and within the cell of interest after exposure to BP nanoflakes. The ATR-FTIR data, coupled with microscopy, reveals chemical changes to the cellular phospholipids, proteins, structural polysaccharides and nucleic acids when compared to untreated cells. These changes can be attributed to the physical interaction combined with the oxidative stress induced by the degradation of the BP nanoflakes. This study provides an insight into the biochemical interaction of BP nanoflakes with microbial cells, allowing for a better understanding of the antimicrobial mechanism of action. en_AU
dc.identifier.citationShaw, Z, L., Cheeseman, S., Huang, L, Z, Y., Peneman, R., Ahmed, T., Bryant, S, J., Christofferson, A, J., Orwell-trigg, R., Dekiwadia, C., Truong, V. K., Vongsvivut, J. P., Walia, S., & Elbourne, A. (2022). Biochemical interaction of few layer black phosphorus with microbial cells using synchrotron macro-ATR-FTIR. Paper presented to CAMS 2022 Advancing Materials and Manufacting, The University of Melbourne, 1st - 3rd June 2022. en_AU
dc.identifier.conferenceenddate3 June 2022en_AU
dc.identifier.conferencenameCAMS 2022: Advancing Materials and Manufacturingen_AU
dc.identifier.conferenceplaceMelbourne, Australiaen_AU
dc.identifier.conferencestartdate1 June 2022en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14591en_AU
dc.language.isoenen_AU
dc.publisherMaterials Australian and The Australian Ceramic Societyen_AU
dc.subjectPhosphorusen_AU
dc.subjectSynchrotronsen_AU
dc.subjectFourier transformationen_AU
dc.subjectDrugsen_AU
dc.subjectProteinsen_AU
dc.subjectPhospholipidsen_AU
dc.titleBiochemical interaction of few layer black phosphorus with microbial cells using synchrotron macro-ATR-FTIRen_AU
dc.typeConference Abstracten_AU
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