Melting of highly oriented fiber DNA subjected to osmotic pressure

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dc.contributor.authorWildes, ARen_AU
dc.contributor.authorKhadeeva, LZen_AU
dc.contributor.authorTrewby, Wen_AU
dc.contributor.authorValle-Orero, Jen_AU
dc.contributor.authorStuder, AJen_AU
dc.contributor.authorGarden, JLen_AU
dc.contributor.authorPeyrard, Men_AU
dc.date.accessioned2021-01-11T03:52:45Zen_AU
dc.date.available2021-01-11T03:52:45Zen_AU
dc.date.issued2015-03-15en_AU
dc.date.statistics2020-01-08en_AU
dc.description.abstractA pilot study of the possibility to investigate temperature-dependent neutron scattering from fiber-DNA in solution is presented. The study aims to establish the feasibility of experiments to probe the influence of spatial confinement on the structural correlation and the formation of denatured bubbles in DNA during the melting transition. Calorimetry and neutron scattering experiments on fiber samples immersed in solutions of poly(ethylene glycol) (PEG) prove that the melting transition occurs in these samples, that the transition is reversible to some degree, and that the transition is broader in temperature than for humidified fiber samples. The PEG solutions apply an osmotic pressure that maintains the fiber orientation, establishing the feasibility of future scattering experiments to study the melting transition in these samples. © 2015 American Chemical Societyen_AU
dc.identifier.citationWildes, A., Khadeeva, L., Trewby, W., Valle-Orero, J., Studer, A., Garden, J.-L., & Peyrard, M. (2015). Melting of highly oriented fiber DNA subjected to osmotic pressure. The Journal of Physical Chemistry B, 119(12), 4441-4449. doi:10.1021/acs.jpcb.5b01343en_AU
dc.identifier.issn1520-6106en_AU
dc.identifier.issue12en_AU
dc.identifier.journaltitleThe Journal of Physical Chemistry Ben_AU
dc.identifier.pagination4441-4449en_AU
dc.identifier.urihttps://doi.org/10.1021/acs.jpcb.5b01343en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/10235en_AU
dc.identifier.volume119en_AU
dc.language.isoenen_AU
dc.publisherACS Publicationsen_AU
dc.subjectMeltingen_AU
dc.subjectFibersen_AU
dc.subjectGeneticsen_AU
dc.subjectMoleculesen_AU
dc.subjectScatteringen_AU
dc.subjectDNAen_AU
dc.subjectPolyethylene glycolsen_AU
dc.titleMelting of highly oriented fiber DNA subjected to osmotic pressureen_AU
dc.typeJournal Articleen_AU
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