Solid and liquid surface-supported bacterial membrane mimetics as a platform for the functional and structural studies of antimicrobials

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dc.contributor.authorLi, Sen_AU
dc.contributor.authorRen, Ren_AU
dc.contributor.authorLyu, Len_AU
dc.contributor.authorSong, JNen_AU
dc.contributor.authorWang, Yen_AU
dc.contributor.authorLin, TWen_AU
dc.contributor.authorBrun, ALen_AU
dc.contributor.authorHsu, HYen_AU
dc.contributor.authorShen, HHen_AU
dc.date.accessioned2024-12-19T03:21:13Zen_AU
dc.date.available2024-12-19T03:21:13Zen_AU
dc.date.issued2022-09-20en_AU
dc.date.statistics2024-11-15en_AU
dc.description.abstractIncreasing antibiotic resistance has provoked the urgent need to investigate the interactions of antimicrobials with bacterial membranes. The reasons for emerging antibiotic resistance and innovations in novel therapeutic approaches are highly relevant to the mechanistic interactions between antibiotics and membranes. Due to the dynamic nature, complex compositions, and small sizes of native bacterial membranes, bacterial membrane mimetics have been developed to allow for the in vitro examination of structures, properties, dynamics, and interactions. In this review, three types of model membranes are discussed: monolayers, supported lipid bilayers, and supported asymmetric bilayers; this review highlights their advantages and constraints. From monolayers to asymmetric bilayers, biomimetic bacterial membranes replicate various properties of real bacterial membranes. The typical synthetic methods for fabricating each model membrane are introduced. Depending on the properties of lipids and their biological relevance, various lipid compositions have been used to mimic bacterial membranes. For example, mixtures of phosphatidylethanolamines (PE), phosphatidylglycerols (PG), and cardiolipins (CL) at various molar ratios have been used, approaching actual lipid compositions of Gram-positive bacterial membranes and inner membranes of Gram-negative bacteria. Asymmetric lipid bilayers can be fabricated on solid supports to emulate Gram-negative bacterial outer membranes. To probe the properties of the model bacterial membranes and interactions with antimicrobials, three common characterization techniques, including quartz crystal microbalance with dissipation (QCM-D), surface plasmon resonance (SPR), and neutron reflectometry (NR) are detailed in this review article. Finally, we provide examples showing that the combination of bacterial membrane models and characterization techniques is capable of providing crucial information in the design of new antimicrobials that combat bacterial resistance. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).en_AU
dc.format.mediumElectronicen_AU
dc.identifier.articlenumber906en_AU
dc.identifier.citationLi, S., Ren, R., Lyu, L., Song, J., Wang, Y., Lin, T.-W., Brun, A. L., Hsu, H.-Y., & Shen, H.-H. (2022). Solid and liquid surface-supported bacterial membrane mimetics as a platform for the functional and structural studies of antimicrobials. Membranes, 12(10), 906. doi:10.3390/membranes12100906en_AU
dc.identifier.issn2077-0375en_AU
dc.identifier.issue10en_AU
dc.identifier.journaltitleMembranesen_AU
dc.identifier.urihttps://doi.org/10.3390/membranes12100906en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15835en_AU
dc.identifier.volume12en_AU
dc.languageEnglishen_AU
dc.language.isoenen_AU
dc.publisherMDPIen_AU
dc.subjectBacteriaen_AU
dc.subjectQuartzen_AU
dc.subjectPlasmonsen_AU
dc.subjectNeutronsen_AU
dc.subjectSolidsen_AU
dc.subjectLiquidsen_AU
dc.subjectMembranesen_AU
dc.subjectAntibioticsen_AU
dc.subjectSurfacesen_AU
dc.titleSolid and liquid surface-supported bacterial membrane mimetics as a platform for the functional and structural studies of antimicrobialsen_AU
dc.typeJournal Articleen_AU
dcterms.dateAccepted2022-09-13en_AU
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