Elemental mapping in a preclinical animal model reveals white matter copper elevation in the acute phase of central nervous system trauma

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dc.contributor.authorEvans, CWen_AU
dc.contributor.authorEgid, Aen_AU
dc.contributor.authorMamsa, SSAen_AU
dc.contributor.authorPaterson, DJen_AU
dc.contributor.authorHo, Den_AU
dc.contributor.authorBartlett, CAen_AU
dc.contributor.authorFehily, Ben_AU
dc.contributor.authorLins, BRen_AU
dc.contributor.authorFitzgerald, Men_AU
dc.contributor.authorHackett, MJen_AU
dc.contributor.authorSmith, NMen_AU
dc.date.accessioned2025-03-21T03:32:13Zen_AU
dc.date.available2025-03-21T03:32:13Zen_AU
dc.date.issued2023-09-11en_AU
dc.date.statistics2024-10-02en_AU
dc.description.abstractUnderstanding the chemical events following trauma to the central nervous system could assist in identifying causative mechanisms and potential interventions to protect neural tissue. Here, we apply a partial optic nerve transection model of injury in rats and use synchrotron X-ray fluorescence microscopy (XFM) to perform elemental mapping of metals (K, Ca, Fe, Cu, Zn) and other related elements (P, S, Cl) in white matter tracts. The partial optic nerve injury model and spatial precision of microscopy allow us to obtain previously unattained resolution in mapping elemental changes in response to a primary injury and subsequent secondary effects. We observed significant elevation of Cu levels at multiple time points following the injury, both at the primary injury site and in neural tissue near the injury site vulnerable to secondary damage, as well as significant changes in Cl, K, P, S, and Ca. Our results suggest widespread metal dyshomeostasis in response to central nervous system trauma and that altered Cu homeostasis may be a specific secondary event in response to white matter injury. The findings highlight metal homeostasis as a potential point of intervention in limiting damage following nervous system injury. © 2023 American Chemical Society.en_AU
dc.description.sponsorshipThis work was supported by the Australian Research Council and the National Health and Medical Research Council of Australia. C.W.E. acknowledges funding by the State Government of Western Australia. S.S.A.M. acknowledges support through a Westpac Future Leaders Scholarship. Part of this research was undertaken on the X-ray Fluorescence Microscopy beamline at the Australian Synchrotron, part of ANSTO, and the authors gratefully acknowledge travel funding provided by ANSTO. This work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). N.M.S. acknowledges the award of an ANSTO─Australian Synchrotron grant and Neurotrauma Research Program Fellowship.en_AU
dc.format.mediumPrint-Electronicen_AU
dc.identifier.citationEvans, C. W., Egid, A., Mamsa, S. S. A., Paterson, D. J., Ho, D., Bartlett, C. A., Fehily, B., Lins, B. R., Fitzgerald, M., Hackett, M. J., & Smith, N. M. (2023). Elemental mapping in a preclinical animal model reveals white matter copper elevation in the acute phase of central nervous system trauma. ACS Chemical Neuroscience, 14(18), 3518-3527. doi:10.1021/acschemneuro.3c00421en_AU
dc.identifier.issn1948-7193en_AU
dc.identifier.issue18en_AU
dc.identifier.journaltitleACS Chemical Neuroscienceen_AU
dc.identifier.pagination3518-3527en_AU
dc.identifier.urihttps://doi.org/10.1021/acschemneuro.3c00421en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/16085en_AU
dc.identifier.volume14en_AU
dc.languageEnglishen_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Societyen_AU
dc.subjectAnimalsen_AU
dc.subjectCopperen_AU
dc.subjectCentral nervous systemen_AU
dc.subjectZincen_AU
dc.subjectCalciumen_AU
dc.subjectPotassiumen_AU
dc.subjectInjuriesen_AU
dc.subjectSynchrotronsen_AU
dc.subjectLipidsen_AU
dc.subjectOpticsen_AU
dc.subjectIonsen_AU
dc.subjectMetalsen_AU
dc.subjectHomeostasisen_AU
dc.subjectOpticsen_AU
dc.titleElemental mapping in a preclinical animal model reveals white matter copper elevation in the acute phase of central nervous system traumaen_AU
dc.typeJournal Articleen_AU
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