Studying biological coordination chemistry: a useful role for low latency, energy-dispersive photon counting XRF detectors

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dc.contributor.authorJames, Sen_AU
dc.contributor.authorde Jonge, MDen_AU
dc.contributor.authorMcColl, Gen_AU
dc.contributor.authorBurke, Ren_AU
dc.contributor.authorPaterson, DJen_AU
dc.contributor.authorHoward, DLen_AU
dc.contributor.authorHare, Den_AU
dc.date.accessioned2022-11-30T01:39:25Zen_AU
dc.date.available2022-11-30T01:39:25Zen_AU
dc.date.issued2016-02-04en_AU
dc.date.statistics2022-11-04en_AU
dc.description.abstractDay to day cellular function is fundamentally dependent on electron transfer reactions mediated by transition metals, often iron and/or copper. The biological consequences of this metal-catalysed redox chemistry arise from biochemical context generated via the multi-scale organisation of biological systems, i.e. the local concentration of metal → the nature of the donor atoms and bonding environment within the ligand → the location and abundance of the ligand within the cell → the suite of metal-ligand complexes comprising a cell’s metallome → the differences between one cell’s instance of it’s metallome compared to another within and between tissues. Biochemical insight must be anchored to the structural biology of the cell. In this view, understanding metallobiology requires us to interrogate the coordination environment of biological metal-ligand complexes in situ, and the lack of suitable probes limits our appreciation for the role metallobiology plays in health and disease. Ideally, such probes must exhibit extremely high specificity, sensitivity, and spatial resolution; requirements met by scanning X-ray fluorescence microscopy (XFM) and X-ray Emission Near Edge Structure (XENES). Advances in energy-dispersive detector technology have enormously enhanced the efficiency and speed of data acquisition when performing XFM and XENES measurements. When using the Maia detector system installed at the Australian Synchrotron XFM beamline the distribution of biometals can be mapped at rates in excess of 3 M pix / hr. This speed reduces imaging dose whilst maintaining counting statistics. Exploiting these technical advances we have undertaken a multi-pronged assault on characterising elemental distribution and speciation in a variety of whole- organism biological systems, including Caenorhabditis elegans and Drosophila melanogaster. We have utilised projective elemental mapping and 3D visualisations of elemental distributions to assess the distribution of chemical speciation through XENES imaging and tomography. The complementarity of these studies demonstrates that volumetric chemical speciation is achievable with the right instrumentation and approach to measurement but projective imaging can still provide a window into fundamental biological processes. Opportunities and challenges associated with visualizing in situ biometal speciation will be discussed.en_AU
dc.identifier.citationJames, S., de Jonge, M., McColl, G., Burke, R., Paterson, D., Howard, D., & Hare, D. (2016). Studying biological coordination chemistry: a useful role for low latency, energy-dispersive photon counting XRF detectors. Paper presented to ACMM24 : Australian Conference on Microscopy and Analysis : Melbourne Convention and Exhibition Centre, 31 Jan-4 Feb 2016. (pp. 29-30).en_AU
dc.identifier.conferenceenddate4 February 2016en_AU
dc.identifier.conferencenameACMM24 : Australian Conference on Microscopy and Analysisen_AU
dc.identifier.conferenceplaceMelbourne, Australiaen_AU
dc.identifier.conferencestartdate31 January 2016en_AU
dc.identifier.isbn9780980337334en_AU
dc.identifier.pagination29-30en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14122en_AU
dc.language.isoenen_AU
dc.publisherAustralian Microscopy and Microanalysis Societyen_AU
dc.subjectChemistryen_AU
dc.subjectPhotonsen_AU
dc.subjectMetalsen_AU
dc.subjectIronen_AU
dc.subjectCopperen_AU
dc.subjectRedox reactionsen_AU
dc.subjectLigandsen_AU
dc.subjectBondingen_AU
dc.subjectFluorescence spectroscopyen_AU
dc.subjectTomographyen_AU
dc.titleStudying biological coordination chemistry: a useful role for low latency, energy-dispersive photon counting XRF detectorsen_AU
dc.typeConference Abstracten_AU
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