Cell size as a primary determinant in targeted nanoparticle uptake

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dc.contributor.authorHoward, Den_AU
dc.contributor.authorTurnbull, Ten_AU
dc.contributor.authorPaterson, DJen_AU
dc.contributor.authorThierry, Ben_AU
dc.contributor.authorKempson, Ien_AU
dc.date.accessioned2024-12-20T02:00:21Zen_AU
dc.date.available2024-12-20T02:00:21Zen_AU
dc.date.issued2022-08-26en_AU
dc.date.statistics2024-10-30en_AU
dc.description.abstractNanoparticle (NP) internalization by cells is complex, highly heterogeneous, and fundamentally important for nanomedicine. We report powerful probabilistic statistics from single-cell data on quantitative NP uptake of PEG-coated transferrin receptor-targeted gold NPs for cancer-derived and fibroblast cells according to their cell size, receptor expression, and receptor density. The smaller cancer cells had a greater receptor density and more efficient uptake of targeted NPs. However, simply due to fibroblasts being larger with more receptors, they exhibited greater NP uptake. While highly heterogeneous, targeted NP uptake strongly correlated with receptor expression. When uptake was normalized to cell size, no correlation existed. Consequently, skewed population distributions in cell sizes explain the distribution in NP uptake. Furthermore, exposure to the transferrin receptor-targeted NPs alters the fibroblast size and receptor expression, suggesting that the receptor-targeted NPs may interfere with the metabolic flux and nutrient exchange, which could assist in explaining the altered regulation of cells exposed to nanoparticles. © 2022 American Chemical Society.en_AU
dc.description.sponsorshipThis research was supported by the Australian Government through the Australian Research Council’s Discovery Projects funding scheme (project no. DP190102119); the X-ray Fluorescence Microscopy beamline at the Australian Synchrotron, part of ANSTO; and the facilities, scientific and technical assistance of Microscopy Australia, funded by the University of South Australia, State and Federal Governments.en_AU
dc.format.mediumPrint-Electronicen_AU
dc.identifier.citationHoward, D., Turnbull, T., Paterson, D. J., Thierry, B., & Kempson, I. (2022). Cell size as a primary determinant in targeted nanoparticle uptake. ACS Applied Bio Materials, 5(9), 4222-4231. doi:10.1021/acsabm.2c00434en_AU
dc.identifier.issn2576-6422en_AU
dc.identifier.issue9en_AU
dc.identifier.journaltitleACS Applied Bio Materialsen_AU
dc.identifier.pagination4222-4231en_AU
dc.identifier.urihttps://doi.org/10.1021/acsabm.2c00434en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15845en_AU
dc.identifier.volume5en_AU
dc.languageEnglishen_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Societyen_AU
dc.subjectNanoparticlesen_AU
dc.subjectReceptorsen_AU
dc.subjectGolden_AU
dc.subjectFluorescenceen_AU
dc.subjectMetabolic diseasesen_AU
dc.subjectNutrientsen_AU
dc.subjectReceptorsen_AU
dc.subjectTherapeutic dosesen_AU
dc.titleCell size as a primary determinant in targeted nanoparticle uptakeen_AU
dc.typeJournal Articleen_AU
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