Investigating the role of Zn in glucose regulation using x-ray fluorescence microscopy and x-ray absorption near-edge structure spectroscopy

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dc.contributor.authorEllison, Gen_AU
dc.contributor.authorBambery, KRen_AU
dc.contributor.authorHackett, MJen_AU
dc.contributor.authorHollings, Aen_AU
dc.contributor.authorHoward, DJen_AU
dc.contributor.authorSharif, Aen_AU
dc.contributor.authorTakechi, RNen_AU
dc.date.accessioned2024-02-28T04:12:07Zen_AU
dc.date.available2024-02-28T04:12:07Zen_AU
dc.date.issued2021-11-24en_AU
dc.date.statistics2023-04-21en_AU
dc.description.abstractZinc plays an important function in glucose regulation, particularly within pancreatic islets, the anatomical home of the glucose regulating hormones insulin and glucagon. Glucose dysregulation is a significant contributor to the epidemic of metabolic diseases, including diabetes, that affect an increasing number of people. Zn is found in very high (mM) concentrations in insulin-secreting β-cells, where it facilitates insulin synthesis and storage, and is co-secreted with insulin, subsequently acting as a signalling molecule. Zn dysregulation is often coincident with impairment of insulin secretion, but little is known about the nature of the changes. Since a subset of the pool of Zn in islets is labile, it is difficult to image in its in vivo situation using conventional techniques such as histochemistry. Not only do preparation steps such as washing displace Zn, but some forms in which it exists are not readily discernible using conventional microscopy techniques. X-ray fluorescence microscopy (XFM) and X-ray absorption near-edge structure spectroscopy (XANES) offer several advantages in that tissue preparation is minimal, facilitating the conservation of native states, and all forms of Zn are not only detectable, but are able to be discriminated by matching spectra against an existing library of Zn forms. Here we report the preliminary results from our study of Zn speciation and elemental mapping in murine islets from healthy or diabetes-prone animals in two age groups, 14 (denoted young) or 28 (old) weeks. This work uses a library of biologically relevant Zn forms created in our laboratory, and contributes to our understanding of the role of Zn in glucose regulation in health and disease, including aging. © The Authorsen_AU
dc.identifier.citationEllison, G., Bambery. K., Hackett, M., Hollings, A., Howard, D., Sharif, A., & Takechi, R. (2021). Investigating the role of Zn in glucose regulation using x-ray fluorescence microscopy and x-ray absorption near-edge structure spectroscopy. Presentation to the ANSTO User Meeting, 24-26 November 2021, Online. Retrieved from: https://events01.synchrotron.org.au/event/146/contributions/4263/en_AU
dc.identifier.conferenceenddate2021-11-26en_AU
dc.identifier.conferencenameANSTO User Meetingen_AU
dc.identifier.conferenceplaceOnlineen_AU
dc.identifier.conferencestartdate2021-11-24en_AU
dc.identifier.urihttps://events01.synchrotron.org.au/event/146/contributions/4263/en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15471en_AU
dc.language.isoenen_AU
dc.publisherAustralian Nuclear Science and Technology Organisationen_AU
dc.subjectZincen_AU
dc.subjectGlucoseen_AU
dc.subjectInsulinen_AU
dc.subjectMetabolic diseasesen_AU
dc.subjectX-ray fluoresence analysisen_AU
dc.subjectAnimalsen_AU
dc.titleInvestigating the role of Zn in glucose regulation using x-ray fluorescence microscopy and x-ray absorption near-edge structure spectroscopyen_AU
dc.typeConference Presentationen_AU
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