X-ray and neutron reflectivity study of the membrane-bound CLIC1 protein at the air-water interface

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dc.contributor.authorHossain, KRen_AU
dc.contributor.authorHolt, SAen_AU
dc.contributor.authorKhamici, HAen_AU
dc.contributor.authorValenzuela, SMen_AU
dc.date.accessioned2021-10-26T22:23:38Zen_AU
dc.date.available2021-10-26T22:23:38Zen_AU
dc.date.issued2016-11-29en_AU
dc.date.statistics2021-10-12en_AU
dc.description.abstractThe CLIC proteins are a ubiquitous family of Chloride Intracellular Ion Channel proteins that are evolutionarily conserved across species and exist as a soluble form and an integral membrane-bound form 1. The X-ray structure of the soluble form of a number of CLIC proteins has been solved and their putative transmembrane domain identified 2-5. However, the factors which facilitate the membrane insertion, the structural transition between these two forms and the structural features of the membrane-bound form for this class of proteins remain largely unknown. In an attempt to answer these questions, we have employed biophysical techniques to study the interaction of wild-type and mutant versions of the protein CLIC1 with monolayers prepared using various mixtures of different phospholipids and sterol molecules. Our findings have demonstrated that cholesterol plays a crucial role for the penetration of CLIC1 into the hydrophobic tails of the lipid monolayer with the protein occupying an area per molecule between 5-7 nm2. We have also demonstrated for the first time that CLIC1 interaction with cholesterol is dependent on the intact 3β-OH group in the sterol ring and that the GXXXG motif in CLIC1 acts as the cholesterol-binding site used by the protein for its initial recognition and binding to membrane cholesterol.en_AU
dc.identifier.citationHossain, K. R., Holt, S. A., Khamici, H. A., & Valenzuela, S. M. (2016). X-ray and neutron reflectivity study of the membrane-bound CLIC1 protein at the air-water interface. Paper presented at 13th AINSE-ANBUG Neutron Scattering Symposium, Sydney, NSW, Australia, 29-30 November 2016.en_AU
dc.identifier.conferenceenddate30 November 2016en_AU
dc.identifier.conferencename13th AINSE-ANBUG Neutron Scattering Symposiumen_AU
dc.identifier.conferenceplaceSydney, NSW, Australiaen_AU
dc.identifier.conferencestartdate29 November 2016en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/12107en_AU
dc.language.isoenen_AU
dc.publisherAustralian Institute of Nuclear Science and Engineeringen_AU
dc.subjectProteinsen_AU
dc.subjectChloridesen_AU
dc.subjectBiophysicsen_AU
dc.subjectCholesterolen_AU
dc.subjectPhospholipidsen_AU
dc.subjectSterolsen_AU
dc.titleX-ray and neutron reflectivity study of the membrane-bound CLIC1 protein at the air-water interfaceen_AU
dc.typeConference Abstracten_AU
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