An intra-pancreatic and hepatic nude mouse cancer xenograft model for boron neutron capture therapy

Mallesch, JL; Chiaraviglio, D; Allen, BJ; Moore, DE

An intra-pancreatic and hepatic nude mouse cancer xenograft model for boron neutron capture therapy

Date

1991-10-02

Authors

Publisher

AINSE

Abstract

The cure of many cancers still depends on early detection and surgical excision. The present protocols for the treatment of hepatic and pancreatic cancers have poor selectivity and this compromises their effectiveness. Our aim has been to establish and then utilise a new experimental model in order to assess the potential of Neutron Capture Therapy for the management of pancreatic and liver carcinomas. Subcutaneous inoculation of pancreatic and hepatic tumour cell lines into the flank of nude mice has many limitations. Inoculation of cancer cells into their organ of origin may produce a different, but potentially more realistic, behaviour of that tumour type. This may then provide a superior indication of the potential of targeting these cancers with boron compounds. Harding-Passey murine melanoma cells were injected between the peritoneal membranes containing the pancreas, or directly into the parenchyma of the right lobe of the liver, in nude mice. One week after inoculation, 12mg of BPA-fructosc was injected intrapcritoneally, then the mice were sacrificed at intervals up to 24 hours. The primary tumour obtained from the inoculation site is a discrete nodule which can be easily separated from the surrounding healthy tissue, providing enough material for boron analysis by ICP-AES. The pancreatic inoculations are very successful and the tumours possess faster growth kinetics than subcutaneously injected control tunours. The pancreas has a greater uptake of BPA-fructose than the tumour, for both models. The hepatic inoculations are less successful and produce smaller tumors but the tumour/liver boron ratio is in the range of 2-4. Because the tumour/pancreas ratio is less than 1 for intrapancrcatic melanoma, hormonal stimulation/ blocking and/or inhibition techniques will be required to reverse this situation. © The Author

Description

Physical copy held by ANSTO Library at DDC: 571.45/15

Keywords

Biological models, Boron compounds, Experimental neoplasms, Liver, Medicine, Mice, Neoplasms, Neutron capture therapy, Organs, Pancreas, Radiotherapy, Rodents, Therapy, Uptake

Citation

Mallesch, J. L., Chiaraviglio, D., Allen, B. J., & Moore, D. E. (1991). An intra-pancreatic and hepatic nude mouse cancer xenograft model for boron neutron capture therapy. Presentation to the 13th AINSE Radiation Biology Conference, 2-4 October 1991, Lucas Heights - AINSE Theatre : conference handbook (programme, abstracts and general information), (pp.23). Lucas Heights, New South Wales : AINSE.