Investigation on the relationship between lipid composition and structure in model membranes composed of extracted natural phospholipids

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dc.contributor.authorSantamaria, Aen_AU
dc.contributor.authorBatchu, KCen_AU
dc.contributor.authorFragneto, Gen_AU
dc.contributor.authorLaux, Ven_AU
dc.contributor.authorHaertlein, Men_AU
dc.contributor.authorDarwish, TAen_AU
dc.contributor.authorRussell, RAen_AU
dc.contributor.authorZaccai, NRen_AU
dc.contributor.authorGuzmán, Een_AU
dc.contributor.authorMaestro, Aen_AU
dc.date.accessioned2023-03-23T04:38:46Zen_AU
dc.date.available2023-03-23T04:38:46Zen_AU
dc.date.issued2023-01-11en_AU
dc.date.statistics2023-03-23en_AU
dc.description.abstractHypothesis Unravelling the structural diversity of cellular membranes is a paramount challenge in life sciences. In particular, lipid composition affects the membrane collective behaviour, and its interactions with other biological molecules. Experiments Here, the relationship between membrane composition and resultant structural features was investigated by surface pressure-area isotherms, Brewster angle microscopy and neutron reflectometry on in vitro membrane models of the mammalian plasma and endoplasmic-reticulum-Golgi intermediate compartment membranes in the form of Langmuir monolayers. Natural extracted yeast lipids were used because, unlike synthetic lipids, the acyl chain saturation pattern of yeast and mammalian lipids are similar. Findings The structure of the model membranes, orthogonal to the plane of the membrane, as well as their lateral packing, were found to depend strongly on their specific composition, with cholesterol having a major influence on the in-plane morphology, yielding a coexistence of liquid-order and liquid-disorder phases. © 2023 Elsevier B.V.en_AU
dc.description.sponsorshipThe authors thank the Institut Laue-Langevin (DOI: 10.5291/ILL-DATA.DIR-215) for financial support and allocation of beamtime, the ILL D-Lab for provision of the hydrogenous and deuterated yeast cells and the Partnership for Soft Condensed Matter (PSCM) for the lab support and provision of lipid extracts (L-Lab). Part of the lipid extraction activity was funded by the ANR/NSF-PIRE project REACT (Research and Education in Active Coatings Technologies for Human Health) and the Ligue for Advanced Neutron Sources (LENS). E.G. and A.M. received financial support from MICINN (grants PID2019-106557GB-C21 and PID2021-129054NA-I00, respectively), N.R.Z. from the Wellcome Trust (grant 207455/Z/17/Z). A. M. also acknowledges the financial support received from the IKUR Strategy under the collaboration agreement between Ikerbasque Foundation and Materials Physics Center on behalf of the Department of Education of the Basque Government. The National Deuteration Facility in Australia is partly funded by The National Collaborative Research Infrastructure Strategy (NCRIS), an Australian Government initiative. We gratefully acknowledge Javier Carrascosa-Tejedor and Giacomo Corucci for the critical reading of the manuscript and the help in handling natural lipid samples.en_AU
dc.identifier.citationSantamaria, A., Batchu, K. C., Fragneto, G., Laux, V., Haertlein, M., Darwish, T. A., Russell, R. A., Zaccai, N. R., Guzmán, E., & Maestro, A. (2023). Investigation on the relationship between lipid composition and structure in model membranes composed of extracted natural phospholipids. Journal of Colloid and Interface Science, 637, 55-66. doi:10.1016/j.jcis.2023.01.043en_AU
dc.identifier.issn0021-9797en_AU
dc.identifier.journaltitleJournal of Colloid and Interface Scienceen_AU
dc.identifier.pagination55-66en_AU
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0021979723000486en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14714en_AU
dc.identifier.volume637en_AU
dc.language.isoenen_AU
dc.publisherElsevier B. V.en_AU
dc.subjectNeutronsen_AU
dc.subjectMicroscopyen_AU
dc.subjectMembranesen_AU
dc.subjectPhospholipidsen_AU
dc.subjectMoleculesen_AU
dc.subjectIsothermsen_AU
dc.titleInvestigation on the relationship between lipid composition and structure in model membranes composed of extracted natural phospholipidsen_AU
dc.typeJournal Articleen_AU
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