Simultaneously localising biometals within the high resolution ultrastructure of whole C. elegans

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dc.contributor.authorJones, MWMen_AU
dc.contributor.authorMcColl, Gen_AU
dc.contributor.authorvan Riessen, GAen_AU
dc.contributor.authorPhillips, NWen_AU
dc.contributor.authorVine, Den_AU
dc.contributor.authorAbbey, Ben_AU
dc.contributor.authorde Jonge, MDen_AU
dc.date.accessioned2023-01-27T00:52:59Zen_AU
dc.date.available2023-01-27T00:52:59Zen_AU
dc.date.issued2016-02-04en_AU
dc.date.statistics2022-11-03en_AU
dc.description.abstractPtychography is a coherent diffraction imaging method where multiple overlapping diffraction frames are combined, providing high resolution images of the electron density of extended objects. Recently, X-ray ptychography has seen many efficiency improvements that allow large areas to be imaged rapidly, making simultaneous X-ray ptychography and fluorescence microscopy experimentally viable. Here we use simultaneous X-ray fluorescence microscopy and ptychography to image entire C. elegans, with sub-micron and sub 100 nm elemental and ultrastructure resolutions respectively. Rapid data collection allowed the entire 1 mm long animal to be imaged in only a few hours. With the information from both techniques, the elemental maps can be viewed in the context of the high resolution ultrastructure, allowing further insights into the localisation of the fluorescent signal.en_AU
dc.identifier.citationJones, M., McColl, G., van Riessen, G., Phillips, N., Vine, D., Abbey, B., & de Jonge, M. (2016). Simultaneously localising biometals within the high resolution ultrastructure of whole C. elegans. Paper presented to ACMM24 : Australian Conference on Microscopy and Analysis : Melbourne Convention and Exhibition Centre, 31 Jan-4 Feb 2016. (pp. 28).en_AU
dc.identifier.conferenceenddate4 February 2016en_AU
dc.identifier.conferencenameACMM24 : Australian Conference on Microscopy and Analysisen_AU
dc.identifier.conferenceplaceMelbourne, Australiaen_AU
dc.identifier.conferencestartdate31 January 2016en_AU
dc.identifier.isbn9780980337334en_AU
dc.identifier.pagination28en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14530en_AU
dc.language.isoenen_AU
dc.publisherAustralian Microscopy and Microanalysis Societyen_AU
dc.subjectMetalsen_AU
dc.subjectUltrastructural changesen_AU
dc.subjectDiffractionen_AU
dc.subjectImagesen_AU
dc.subjectX-ray equipmenten_AU
dc.subjectFluorescence spectroscopyen_AU
dc.subjectFluorescenceen_AU
dc.titleSimultaneously localising biometals within the high resolution ultrastructure of whole C. elegansen_AU
dc.typeConference Abstracten_AU
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