Subcellular distribution of the 18 kDa translocator protein and transcript variant PBR-S in human cells

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dc.contributor.authorLiu, GJen_AU
dc.contributor.authorMiddleton, RJen_AU
dc.contributor.authorBanati, RBen_AU
dc.date.accessioned2022-08-29T02:20:27Zen_AU
dc.date.available2022-08-29T02:20:27Zen_AU
dc.date.issued2017-05-20en_AU
dc.date.statistics2022-08-04en_AU
dc.description.abstractDespite continued interest in the 18 kDa translocator protein (PBR/TSPO) as a biomarker and a therapeutic target for a range of diseases, its functional role, such as in the steroid synthesis pathway and energy metabolism has either become contentious or remains to be described more precisely. The PBR/TSPO gene consists of four exons, while a shorter isoform termed PBR-S lacks exon 2. The PBR-S 102-codon open reading frame differs to that of PBR/TSPO, resulting in a protein that is completely unrelated to PBR/TSPO. To our knowledge, PBR-S protein has never been described and has no known or proposed function. To obtain possible clues on the role of this uncharacterised protein, we compared the subcellular distribution of PBR-S to that of PBR/TSPO. By expressing fluorescently tagged PBR/TSPO, we confirmed that full-length PBR/TSPO co-localises with mitochondria in HeLa, HEK-293, MDA-MB-231, BJ and U87-MG human cell lines. Unlike the strictly mitochondrial localisation of PBR/TSPO, PBR-S has a punctate distribution throughout the cytosol that co-localises with lysosomes in HeLa, HEK-293, MDA-MB-231, BJ and U87-MG cells. In summary, within the cell lines examined we confirm mitochondria rather than occasionally reported other localisations, such as the cell nucleus, to be the only site where PBR/TSPO resides. Due to the lack of any shared protein sequences and the different subcellular locations, we suggest that the previously uncharacterised PBR-S protein variant of the PBR/TSPO gene is likely to serve a different yet to be discovered function compared to PBR/TSPO. © 2017 Elsevier B.Ven_AU
dc.description.sponsorshipThe author's acknowledge the support from the ANSTO Distinguished Research Fellowship held by Richard B. Banati.en_AU
dc.identifier.citationLiu, G.-J., Middleton, R. J., & Banati, R. B. (2017). Subcellular distribution of the 18 kDa translocator protein and transcript variant PBR-S in human cells. Gene, 613, 45-56. doi:10.1016/j.gene.2017.02.035en_AU
dc.identifier.issn0378-1119en_AU
dc.identifier.journaltitleGeneen_AU
dc.identifier.pagination45-56en_AU
dc.identifier.urihttps://doi.org/10.1016/j.gene.2017.02.035en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/13642en_AU
dc.identifier.volume613en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectAnimal cellsen_AU
dc.subjectProteinsen_AU
dc.subjectTranscription factorsen_AU
dc.subjectMitochondriaen_AU
dc.subjectLysosomesen_AU
dc.subjectReceptorsen_AU
dc.subjectDrugsen_AU
dc.titleSubcellular distribution of the 18 kDa translocator protein and transcript variant PBR-S in human cellsen_AU
dc.typeJournal Articleen_AU
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