In vivo deuteration strategies for neutron scattering analysis of bacterial polyhydroxyoctanoate

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dc.contributor.authorRussell, RAen_AU
dc.contributor.authorHolden, PJen_AU
dc.contributor.authorWilde, KLen_AU
dc.contributor.authorGarvey, CJen_AU
dc.contributor.authorHammerton, KMen_AU
dc.contributor.authorFoster, LJRen_AU
dc.date.accessioned2024-02-23T00:17:22Zen_AU
dc.date.available2024-02-23T00:17:22Zen_AU
dc.date.issued2008-05-15en_AU
dc.date.statistics2024-02-19en_AU
dc.description.abstractThe cultivation of microorganisms on deuterated substrates has allowed us to control deuterium incorporation into biopolymer systems which is important for characterisation using neutron scattering techniques. Bacterial polyhydroxyoctanoate (PHO) is a polyester formed within inclusions inside bacterial cells and was deuterated in vivo under various conditions to characterise the formation of these inclusions by neutron scattering. Manipulation of deuterated media during microbial growth and PHO production phases resulted in polymer with partial or complete substitution of hydrogen by deuterium, as shown by gas chromatography. Sequential feeding of hydrogenated and deuterated forms of the same precursor was used to demonstrate that neutron scattering analysis could be used to differentiate between chemically similar phases in these polymer inclusions. © 2008 Crown Copyrighten_AU
dc.description.sponsorshipThis work utilised facilities supported in part by the National Science Foundation under Agreement No. DMR-0454672. We acknowledge the support of the National Institute of Standards and Technology, U.S. Department of Commerce, in providing the neutron research facilities used in this work. The assistance of Man-Ho Kim (Korea Institute of Science and Technology) for USANS data analysis, Andrew Whitten (Bragg, ANSTO) for density measurements and reviewers for their helpful comments is gratefully acknowledged.en_AU
dc.identifier.citationRussell, R. A., Holden, P. J., Wilde, K. L., Garvey, C. J., Hammerton, K. M., & Foster, L. J. R. (2008). In vivo deuteration strategies for neutron scattering analysis of bacterial polyhydroxyoctanoate. European Biophysics Journal, 37, 711-715. doi:10.1007/s00249-008-0333-9en_AU
dc.identifier.issn1432-1017en_AU
dc.identifier.journaltitleEuropean Biophysics Journalen_AU
dc.identifier.pagination711-715en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15402en_AU
dc.identifier.volume37en_AU
dc.language.isoenen_AU
dc.publisherSpringer Natureen_AU
dc.relation.urihttps://doi.org/10.1007/s00249-008-0333-9en_AU
dc.subjectDeuterationen_AU
dc.subjectBacteriaen_AU
dc.subjectNeutron diffractionen_AU
dc.subjectIn vivoen_AU
dc.subjectShell Modelsen_AU
dc.subjectMicroorganismsen_AU
dc.subjectOrganic polymersen_AU
dc.titleIn vivo deuteration strategies for neutron scattering analysis of bacterial polyhydroxyoctanoateen_AU
dc.typeJournal Articleen_AU
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