Small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) study on the structure of sodium caseinate in dispersions and at the oil-water interface: effect of calcium ions

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dc.contributor.authorCheng, LRen_AU
dc.contributor.authorYe, AQen_AU
dc.contributor.authorYang, Zen_AU
dc.contributor.authorGilbert, EPen_AU
dc.contributor.authorKnott, RBen_AU
dc.contributor.authorde Campo, Len_AU
dc.contributor.authorStorer, Ben_AU
dc.contributor.authorHemar, Yen_AU
dc.contributor.authorSingh, Hen_AU
dc.date.accessioned2023-12-01T00:42:39Zen_AU
dc.date.available2023-12-01T00:42:39Zen_AU
dc.date.issued2022-04en_AU
dc.date.statistics2022-05-27en_AU
dc.description.abstractThe structure of sodium caseinate particles, as affected by the presence of calcium ions (Ca2+), in aqueous solution and in oil (toluene)-in-water emulsions, was investigated by small-angle X-ray and neutron scattering (SAXS and SANS). SAXS analyses indicated that the sodium caseinate dispersed in water as small particles with electrostatic interactions, which has a radius of gyration (Rg) of ~5 nm and an effective radius (Reff) of ~ 10 nm with an assuming spherical shape. In the presence of Ca2+, the caseinate particles aggregated as large particles with a hydrodynamic diameter > 100 nm as determined by dynamic light scattering. The networks within the large particles were self-assembled from the small Ca2+-cross-linked particles (Rg ~ 6.5–8.0 nm), as probed by SAXS. The fractal-like dimension increased from 2.5 to 3.4 with increasing protein and CaCl2 concentrations, suggesting a denser structure. The integrity of the caseinate particles at the oil-water interface was enhanced by Ca2+ cross-linking, as observed by transmission electron microscopy. The oilsingle bondwater interface stabilised by Ca2+-cross-linked caseinate particles was ~ 30 nm thick, six times thicker than that stabilised by sodium caseinate (~ 5 nm) as analysed by SANS with contrast variation technique. Quantifying the structure of sodium caseinate in an aqueous solution and at the oil-water interface provides valuable insights for designing new casein-based functional materials. © 2022 Elsevier Ltden_AU
dc.description.sponsorshipThis work was funded by the Riddet Institute, a National Centre of Research Excellence (CoRE), funded by the New Zealand Tertiary Education Commission, New Zealand. We acknowledge the grants and the support of the Australian Nuclear Science and Technology Organization, Australia, for access to the small-angle X-ray scattering (Bruker) and small-angle neutron scattering (QUOKKA) facilities under proposal P8623. We also thank the Manawatu Microscopy and Imaging Centre at Massey University for technical support.en_AU
dc.identifier.articlenumber100276en_AU
dc.identifier.citationCheng, L., Ye, A., Yang, Z., Gilbert, E. P., Knott, R., de Campo, L., Storer, B., Hemar, Y., & Singh, H. (2022). Small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) study on the structure of sodium caseinate in dispersions and at the oil-water interface: effect of calcium ions. Food Structure, 32, 100276. doi:10.1016/j.foostr.2022.100276en_AU
dc.identifier.issn2213-3291en_AU
dc.identifier.journaltitleFood Structureen_AU
dc.identifier.urihttps://doi.org/10.1016/j.foostr.2022.100276en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15256en_AU
dc.identifier.volume32en_AU
dc.language.isoenen_AU
dc.publisherElsevier B. V.en_AU
dc.relation.urihttps://doi.org/10.1016/j.foostr.2022.100276en_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectSodiumen_AU
dc.subjectEmulsionsen_AU
dc.subjectDispersionsen_AU
dc.subjectWateren_AU
dc.subjectParticlesen_AU
dc.subjectInteractionsen_AU
dc.subjectAqueous solutionsen_AU
dc.subjectNeutron diffractionen_AU
dc.titleSmall-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) study on the structure of sodium caseinate in dispersions and at the oil-water interface: effect of calcium ionsen_AU
dc.typeJournal Articleen_AU
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