Fluorescence-activated cell sorting to reveal the cell origin of radioligand binding

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dc.contributor.authorTournier, BBen_AU
dc.contributor.authorTsartsalis, Sen_AU
dc.contributor.authorCeyzériat, Ken_AU
dc.contributor.authorMedina, Zen_AU
dc.contributor.authorFraser, BHen_AU
dc.contributor.authorGrégoire, MCen_AU
dc.contributor.authorKövari, Een_AU
dc.contributor.authorMillet, Pen_AU
dc.date.accessioned2021-09-07T03:16:57Zen_AU
dc.date.available2021-09-07T03:16:57Zen_AU
dc.date.issued2019-06-19en_AU
dc.date.statistics2021-09-07en_AU
dc.description.abstractMany studies have explored the role of TSPO (18 kDa translocator protein) as a marker of neuroinflammation using single-photon emission computed tomography (SPECT) or positron emission tomography (PET). In vivo imaging does not allow to determine the cells in which TSPO is altered. We propose a methodology based on fluorescence-activated cell sorting to sort different cell types of radioligand-treated tissues. We compared left/right hippocampus of rats in response to a unilateral injection of lipopolysaccharide (LPS), ciliary neurotrophic factor (CNTF) or saline. We finally applied this methodology in human samples (Alzheimer's disease patients and controls). Our data show that the pattern of TSPO overexpression differs across animal models of acute neuroinflammation. LPS induces a microglial expansion and an increase in microglial TSPO binding. CNTF is associated with an increase in TSPO binding in microglia and astrocytes in association with an increase in the number of microglial binding sites per cell. In humans, we show that the increase in CLINDE binding in Alzheimer's disease concerns microglia and astrocytes in the presence of a microglial expansion. Thus, the cellular basis of TSPO overexpression is condition dependent, and alterations in TSPO binding found in PET/SPECT imaging studies cannot be attributed to particular cell types indiscriminately. © 2021 International Society for Cerebral Blood Flow and Metabolismen_AU
dc.identifier.citationTournier, B. B., Tsartsalis, S., Ceyzériat, K., Medina, Z., Fraser, B. H., Grégoire, M. C., Kövari, E. & Millet, P. (2020). Fluorescence-activated cell sorting to reveal the cell origin of radioligand binding. Journal of Cerebral Blood Flow & Metabolism, 40(6), 1242-1255. doi:10.1177/0271678X19860408en_AU
dc.identifier.issn1559-7016en_AU
dc.identifier.issue6en_AU
dc.identifier.journaltitleJournal of Cerebral Blood Flow & Metabolismen_AU
dc.identifier.pagination1242-1255en_AU
dc.identifier.urihttps://doi.org/10.1177/0271678X19860408en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/11614en_AU
dc.identifier.volume40en_AU
dc.language.isoenen_AU
dc.publisherSAGEen_AU
dc.subjectNervous system diseasesen_AU
dc.subjectFluorescenceen_AU
dc.subjectInflammationen_AU
dc.subjectPositron computed tomographyen_AU
dc.subjectSingle photon emission computed tomographyen_AU
dc.subjectProteinsen_AU
dc.subjectLigandsen_AU
dc.titleFluorescence-activated cell sorting to reveal the cell origin of radioligand bindingen_AU
dc.typeJournal Articleen_AU
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