Hierarchical assembly of tryptophan zipper peptides into stress-relaxing bioactive hydrogels

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dc.contributor.authorNguyen, AKen_AU
dc.contributor.authorMolley, TGen_AU
dc.contributor.authorKardia, Een_AU
dc.contributor.authorGanda, Sen_AU
dc.contributor.authorChakraborty, Sen_AU
dc.contributor.authorWong, SLen_AU
dc.contributor.authorRuan, JFen_AU
dc.contributor.authorYee, BEen_AU
dc.contributor.authorMata, JPen_AU
dc.contributor.authorVijayan, Aen_AU
dc.contributor.authorKumar, Nen_AU
dc.contributor.authorTilley, RDen_AU
dc.contributor.authorWaters, SAen_AU
dc.contributor.authorKilian, KAen_AU
dc.date.accessioned2023-11-09T02:56:45Zen_AU
dc.date.available2023-11-09T02:56:45Zen_AU
dc.date.issued2023-10-23en_AU
dc.date.statistics2023-11-06en_AU
dc.description.abstractSoft materials in nature are formed through reversible supramolecular assembly of biological polymers into dynamic hierarchical networks. Rational design has led to self-assembling peptides with structural similarities to natural materials. However, recreating the dynamic functional properties inherent to natural systems remains challenging. Here we report the discovery of a short peptide based on the tryptophan zipper (trpzip) motif, that shows multiscale hierarchical ordering that leads to emergent dynamic properties. Trpzip hydrogels are antimicrobial and self-healing, with tunable viscoelasticity and unique yield-stress properties that allow immediate harvest of embedded cells through a flick of the wrist. This characteristic makes Trpzip hydrogels amenable to syringe extrusion, which we demonstrate with examples of cell delivery and bioprinting. Trpzip hydrogels display innate bioactivity, allowing propagation of human intestinal organoids with apical-basal polarization. Considering these extensive attributes, we anticipate the Trpzip motif will prove a versatile building block for supramolecular assembly of soft materials for biotechnology and medicine. © 2023 Springer Nature Limited. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.en_AU
dc.description.sponsorshipA.K.N. acknowledges scholarship support from the Australian Government Research Training Program and the Baxter Family Postgraduate Scholarship. This work was supported through funding from the Australian Research Council Grant FT180100417 (K.A.K.), the National Health and Medical Research Council Grant APP1185021 (K.A.K.) and APP1188987 (S.A.W.), the National Cancer Institute of the National Institutes of Health Grant R01CA251443 (K.A.K.), and the Sydney Children Hospital Network Foundation (S.A.W.) and Luminesce Alliance Research grants (S.A.W.). We thank the study participants and their families for their contributions. We also thank Sydney Children’s Hospitals (SCH) Randwick Cystic Fibrosis clinic especially Prof Adam Jaffe, A/Prof Keith Ooi, Dr Laura Fawcett, Dr Yvonne Belessis, Leanne Plush, Amanda Thompson and Rhonda Bell in the organization and collection of participant biospecimens for miCF biobank. The authors acknowledge the help and support of staff at the Katharina Gaus Light Imaging Facility (KGLMF) of the UNSW Mark Wainwright Analytical Centre. The authors acknowledge the use of the Cryo Electron Microscopy Facility through the Victor Chang Cardiac Research Institute Innovation Centre, funded by the NSW government and the Electron Microscope Unit at UNSW Sydney. This study used the computational cluster Katana supported by Research Technology Services at UNSW Sydney. We would also like to thank the Australian Nuclear Science and Technology Organisation (ANSTO) for providing USANS and SANS beam facilities under proposal number P 14142 for this work.en_AU
dc.identifier.articlenumber6604en_AU
dc.identifier.citationNguyen, A. K., Molley, T. G., Kardia, E., Ganda, S., Chakraborty, S., Wong, S. L., Ruan, J., Yee, B. E., Mata, J., Vijayan, A., Kumar, N., Tilley, R. D., Waters, S. A., & Kilian, K. A. (2023). Hierarchical assembly of tryptophan zipper peptides into stress-relaxing bioactive hydrogels. Nature Communications, 14(1), 6604. doi:10.1038/s41467-023-41907-1en_AU
dc.identifier.issn2041-1723en_AU
dc.identifier.issue1en_AU
dc.identifier.journaltitleNature Communicationsen_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15190en_AU
dc.identifier.volume14en_AU
dc.language.isoenen_AU
dc.publisherSpringer Natureen_AU
dc.relation.urihttps://doi.org/10.1038/s41467-023-41907-1en_AU
dc.subjectTryptophanen_AU
dc.subjectPeptidesen_AU
dc.subjectHydrogelsen_AU
dc.subjectPolymersen_AU
dc.subjectDynamicsen_AU
dc.subjectBiotechnologyen_AU
dc.subjectMedicineen_AU
dc.titleHierarchical assembly of tryptophan zipper peptides into stress-relaxing bioactive hydrogelsen_AU
dc.typeJournal Articleen_AU
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