Evaluation of the PBR ligand [123I]CLINDE in an animal model of experimental autoimmune encephalomyelitis

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dc.contributor.authorMattner, Fen_AU
dc.contributor.authorLinares, Den_AU
dc.contributor.authorStaykova, Men_AU
dc.contributor.authorGrégoire, MCen_AU
dc.contributor.authorPham, TQen_AU
dc.contributor.authorBourdier, Ten_AU
dc.contributor.authorQuinlivan, Men_AU
dc.contributor.authorCallaghan, PDen_AU
dc.contributor.authorWillenborg, DOen_AU
dc.contributor.authorKatsifis, Aen_AU
dc.date.accessioned2023-06-23T02:09:48Zen_AU
dc.date.available2023-06-23T02:09:48Zen_AU
dc.date.issued2008-05-01en_AU
dc.date.statistics2023-01-30en_AU
dc.description.abstractObjectives: The aim of this study was to evaluate the Peripheral Benzodiazepine Receptor (PBR) radioligand [123I]CLINDE in the rat inflammatory disease model of Experimental Autoimmune Encephalomyelitis (EAE). Methods: EAE was induced with blast cells collected from spleen and lymph nodes of Lewis rats induced with myelin basic protein and complete Freund's adjuvant. Biodistribution with [123I]CLINDE was undertaken on EAE rats exhibiting different disease severity and compared to controls.The relationship between inflammatory lesions and tracer uptake was investigated using ex vivo autoradiography and immunohistochemistry. Results: Disease severity was confirmed by histopathology in spinal cord. Results indicate enhanced uptake of [123I]CLINDE in all animals induced with EAE compared to controls. This uptake reflected the ascending nature of the inflammatory lesions ie. uptake in the lumbar spinal cord > thoracic cord > cervical cord > medulla > cerebellum. Uptake of [123I]CLINDE in the lumbar and thoracic cord correlated with disease severity. A 2 and 3 fold enhancement in PBR expression was observed in the brain and spinal cord of animals with a clinical score of 3 compared to controls. Regional [123I]CLINDE uptake closely correlated with localisation of PBR, shown using autoradiography and immunohistochemisty. Conclusions: These results demonstrate the ability of [123I]CLINDE to measure in vivo changes of PBR density according to area of involvement and the severity of disease suggesting it as a potential SPECT tracer for the study of inflammation and multiple sclerosis. © 2022 Journal of Nuclear Medicineen_AU
dc.identifier.citationMattner, F., Linares, D., Staykova, M., Gregoire, M.-C., Pham, T., Bourdier, T., Quinlivan, M., Callaghan, P., Willenborg, D., & Katsifis, A. (2008). Evaluation of the PBR ligand [123I]CLINDE in an animal model of experimental autoimmune encephalomyelitis. Paper presented to the Society of Nuclear Medicine (SNM) 2008 Annual Meeting, New Orleans, Louisiana, USA, June 14 - 18, 2008. In Journal of Nuclear Medicine, 49, (Supplement 1), 81P. Retireved from: http://jnm.snmjournals.org/content/49/supplement_1/81P.1.abstracten_AU
dc.identifier.conferenceenddate18 June 2008en_AU
dc.identifier.conferencenameSociety of Nuclear Medicine (SNM) 2008 Annual Meetingen_AU
dc.identifier.conferenceplaceNew Orleans, Louisianaen_AU
dc.identifier.conferencestartdate14 June 2008en_AU
dc.identifier.issn2159-662Xen_AU
dc.identifier.issueS1en_AU
dc.identifier.journaltitleJournal of Nuclear Medicineen_AU
dc.identifier.pagination81Pen_AU
dc.identifier.urihttps://jnm.snmjournals.org/content/49/supplement_1/81P.1en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15063en_AU
dc.identifier.volume49en_AU
dc.language.isoenen_AU
dc.publisherAustralasian Quaternary Associationen_AU
dc.subjectLigandsen_AU
dc.subjectRatsen_AU
dc.subjectAnimal cellsen_AU
dc.subjectProteinsen_AU
dc.subjectLymph nodesen_AU
dc.subjectFreunds adjuvanten_AU
dc.subjectDiseasesen_AU
dc.subjectImmune system diseasesen_AU
dc.titleEvaluation of the PBR ligand [123I]CLINDE in an animal model of experimental autoimmune encephalomyelitisen_AU
dc.typeConference Abstracten_AU
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