The response of corals and the coral microbiome to metal exposure

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dc.contributor.authorGissi, Fen_AU
dc.contributor.authorReichelt-Brushett, AJen_AU
dc.contributor.authorChariton, AAen_AU
dc.contributor.authorStauber, JLen_AU
dc.contributor.authorStephenson, SAen_AU
dc.contributor.authorCresswell, Ten_AU
dc.contributor.authorGreenfield, Pen_AU
dc.contributor.authorSeverati, Aen_AU
dc.contributor.authorHumphrey, Cen_AU
dc.contributor.authorJolley, DFen_AU
dc.date.accessioned2021-09-13T21:48:42Zen_AU
dc.date.available2021-09-13T21:48:42Zen_AU
dc.date.issued2017-11-12en_AU
dc.date.statistics2021-09-13en_AU
dc.description.abstractThe mining and production of Ni is increasing in tropical regions. The potential impacts of these activities on the valuable coastal ecosystems are poorly understood. Specifically, there is little information available on the effects of Ni to corals. Scleractinian corals are keystone species for coral reefs forming vital structural habitats that support other species, resulting in habitats with high species richness and diversity. For these reasons, it is important that future research provides data which can inform the sustainable development of Ni operations in tropical regions. This study aimed to investigate the effect of dissolved Ni exposure to the scleractinian coral Acropora muricata. Utilising the facilities at the National Sea Simulator (SeaSim), flow through chambers (2.5L) were used to test the effects of Ni and Cu on adult corals and its associated microbiota. Copper was tested alongside Ni to allow for comparisons with past studies. Four replicate chambers were used for; control, 50, 100, 500, 1000, 10000 µg/L Ni and 5, 20, 50, 100 µg/L Cu. Each replicate chamber contained 3 coral fragments (5-8cm in length). After a 96-h exposure, 1 fragment from each chamber was sacrificed for 3 different analytical purposes. One replicate was air blasted to remove tissues which were flash frozen and later used for DNA and RNA sequencing of the microbiota to observe if the bacterial community structure changed in response to metal exposure. A second fragment was air blasted to remove tissues, which were then acid digested and analysed by ICP-MS to determine metal concentrations in the coral tissues. A third replicate was frozen for subsequent metal uptake and distribution analyses using elemental mapping techniques including CT scanning and XRF-ITRAX. Control treatments remained healthy throughout the exposure. After 36 h, bleaching was observed in corals exposed to 50 and 100 µg Cu/L and 10000 µg Ni/L. At 96 h significant discolouration of corals was observed in Ni treatments 500 and 1000 µg Ni/L. The effects of Cu and Ni on adult corals and associated microbiota will be discussed.en_AU
dc.identifier.citationGissi, F., Reichelt-Brushett, A., Chariton, A. A., Stauber, J. L., Stephenson, S., Cresswell, T., Greenfield, P., Severati, A., Humphrey, C., & Jolley, D. F. (2017). The response of corals and the coral microbiome to metal exposure. Paper presented at SETAC North America 38th Annual Meeting, Minneapolis, Minnesota, USA, 12 to 16 November 2017. Retrieved from: https://www.setac.org/events/EventDetails.aspx?id=489412en_AU
dc.identifier.conferenceenddate16 November 2017en_AU
dc.identifier.conferencenameSETAC North America 38th Annual Meetingen_AU
dc.identifier.conferenceplaceMinneapolis, Minnesota, USAen_AU
dc.identifier.conferencestartdate12 November 2017en_AU
dc.identifier.urihttps://www.setac.org/events/EventDetails.aspx?id=489412en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/11675en_AU
dc.language.isoenen_AU
dc.publisherSociety of Environmental Toxicology and Chemistryen_AU
dc.subjectCoralsen_AU
dc.subjectCoral reefsen_AU
dc.subjectTropical regionsen_AU
dc.subjectEcosystemsen_AU
dc.subjectSpecies diversityen_AU
dc.subjectNickelen_AU
dc.subjectMetalsen_AU
dc.titleThe response of corals and the coral microbiome to metal exposureen_AU
dc.typeConference Abstracten_AU
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