Synchrotron infrared micro-spectroscopy of single cells at the Australian Synchrotron

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dc.contributor.authorBambery, KRen_AU
dc.contributor.authorTobin, MJen_AU
dc.contributor.authorPuskar, Len_AU
dc.contributor.authorMartin, Den_AU
dc.contributor.authorVongsvivut, JPen_AU
dc.date.accessioned2023-01-24T01:05:06Zen_AU
dc.date.available2023-01-24T01:05:06Zen_AU
dc.date.issued2016-02-04en_AU
dc.date.statistics2022-11-04en_AU
dc.description.abstractInfrared Microspectroscopy is increasingly revealing valuable bio-chemical information of biological and biomedical systems beyond the tissue level at the single cell level. At the Australian Synchrotron Infrared Microscopy beamline, FTIR spectroscopy provides sensitive molecular fingerprinting for tissues and cells without the need for sample pre-treatment with stains or external markers. Due to the brightness of a synchrotron source, good signal to noise at high spatial resolution (diffraction limited) can routinely be performed at the single cell level. In the study of live microbiological systems the principal restriction on the application of infrared microspectroscopy is the strong absorbance by water in the region of 1650 cm-1, overlaying the Amide I absorption band of proteins. The combination of a highly focused synchrotron beam with liquid cells constructed with microfabricated spacers of 6 to 8 microns in thickness have enabled complete mid-IR spectra to be obtained of single live cells under aqueous media within short scan times. Some applications include analysis of spectral changes in normal single living cells, diagnosing different disease states, discrimination of cell types and monitoring the effects of drug treatment at the single cell level. Details of these studies conducted at the infrared microscopy beamline at the Australian Synchrotron are presented.en_AU
dc.identifier.citationBambery, K. R., Tobin, M. J., Puskar, L., Martin, D., & Vongsvivut, J. (2016). Synchrotron infrared micro-spectroscopy of single cells at the Australian Synchrotron. Paper presented to ACMM24 : Australian Conference on Microscopy and Analysis : Melbourne Convention and Exhibition Centre, 31 Jan-4 Feb 2016, (pp. 35).en_AU
dc.identifier.conferenceenddate4 February 2016en_AU
dc.identifier.conferencenameACMM24 : Australian Conference on Microscopy and Analysisen_AU
dc.identifier.conferenceplaceMelbourne, Australiaen_AU
dc.identifier.conferencestartdate31 January 2016en_AU
dc.identifier.isbn9780980337334en_AU
dc.identifier.pagination35en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14474en_AU
dc.language.isoenen_AU
dc.publisherAustralian Microscopy and Microanalysis Societyen_AU
dc.subjectSynchrotronsen_AU
dc.subjectANSTOen_AU
dc.subjectSpectroscopyen_AU
dc.subjectMolecular beamsen_AU
dc.subjectInfrared spectrometersen_AU
dc.subjectWateren_AU
dc.subjectDiffractionen_AU
dc.subjectAnimal cellsen_AU
dc.titleSynchrotron infrared micro-spectroscopy of single cells at the Australian Synchrotronen_AU
dc.typeConference Abstracten_AU
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