Demonstration of the use of Scenedesmus and Carteria biomass to drive bacterial sulfate reduction by Desulfovibrio alcoholovorans isolated from an artificial wetland

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dc.contributor.authorRussell, RAen_AU
dc.contributor.authorHolden, PJen_AU
dc.contributor.authorWilde, KLen_AU
dc.contributor.authorNeilan, BAen_AU
dc.date.accessioned2024-02-22T23:27:33Zen_AU
dc.date.available2024-02-22T23:27:33Zen_AU
dc.date.issued2003-10en_AU
dc.date.statistics2024-02-20en_AU
dc.description.abstractA major factor limiting application of bacterial sulfate reduction to removal of sulfate and heavy metals in wetland systems is the requirement to supply carbon and energy to drive the process. Primary production by aquatic plants and algae is a cheap option for driving sustainable bacterial sulfate reduction and most operational systems have relied on plants. The use of harvested, non-growing algal biomass to support bacterial sulfate reduction was investigated. Two genera of green algae, strains N9 and A3, were isolated from treatment cells from the Artificial Wetland Filter at the Ranger uranium mine (Northern Territory, Australia) which successfully removes UO22+, Mn2+ and nitrate, but little sulfate, from mine waters. These algae were identified as Carteria sp. and Scenedesmus sp. and were used as the sole carbon and energy source to enrich a sulfate-reducing mixed bacterial culture from the constructed wetland. Bacterial sulfate reduction supported solely by degradation of algal biomass was demonstrated at laboratory scale using both algae. In excess of 300 mg/L, sulfate was reduced in 17 days following an initial period of approximately 8 days during which sulfate levels did not decrease. The amount and rate of reduction was shown to be dependent on the concentration of algal biomass added. Carteria algae at low concentration showed reduction earlier; however, yields at higher concentration were affected by unknown inhibition. Scenedesmus strain N9 produced a maximum specific yield of 94.3 g of sulfate reduced per gram biomass added compared with 43.5 for Carteria strain A3. Sequence analysis of the 16S rRNA gene of members of the bacterial consortium indicated that the sulfate-reducing bacteria (SRB) showed highest homology (98.5%) with Desulfovibrio alcoholovorans. A second bacterium, which showed homologies of 91–92% with organisms of the Clostridial assemblage, was also present in the culture and represents a new species, or possibly a new genus. Crown Copyright 2003 Published by Elsevier B.V.en_AU
dc.description.sponsorshipThe assistance of John M. Ferris in the identification of the algal isolates is gratefully acknowledged. Determination of sulfate concentration of samples by Ion Chromatography was conducted by Agness Knapik.en_AU
dc.identifier.citationRussell, R. A., Holden, P. J., Wilde, K. L., & Neilan, B. A. (2003). Demonstration of the use of Scenedesmus and Carteria biomass to drive bacterial sulfate reduction by Desulfovibrio alcoholovorans isolated from an artificial wetland. Hydrometallurgy, 71(1-2), 227-234. doi:10.1016/S0304-386X(03)00160-9en_AU
dc.identifier.issn0304-386Xen_AU
dc.identifier.issue1-2en_AU
dc.identifier.journaltitleHydrometallurgyen_AU
dc.identifier.pagination227-234en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15396en_AU
dc.identifier.volume71en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.relation.urihttps://doi.org/10.1016/S0304-386X(03)00160-9en_AU
dc.subjectBiomassen_AU
dc.subjectSulfate-reducing Bacteriaen_AU
dc.subjectWetlandsen_AU
dc.subjectAlgaeen_AU
dc.subjectCarbonen_AU
dc.subjectOrganic matteren_AU
dc.subjectHeavy metalsen_AU
dc.subjectMicroorganismsen_AU
dc.titleDemonstration of the use of Scenedesmus and Carteria biomass to drive bacterial sulfate reduction by Desulfovibrio alcoholovorans isolated from an artificial wetlanden_AU
dc.typeJournal Articleen_AU
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