Gold nanoparticle adsorption alters the cell stiffness and cell wall bio-chemical landscape of Candida albicans fungal cells

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dc.contributor.authorPenman, Ren_AU
dc.contributor.authorKariuki, Ren_AU
dc.contributor.authorShaw, ZLen_AU
dc.contributor.authorDekiwadia, Cen_AU
dc.contributor.authorChristofferson, AJen_AU
dc.contributor.authorBryant, Gen_AU
dc.contributor.authorVongsvivut, JPen_AU
dc.contributor.authorBryant, SJen_AU
dc.contributor.authorElbourne, Aen_AU
dc.date.accessioned2024-01-12T00:29:28Zen_AU
dc.date.available2024-01-12T00:29:28Zen_AU
dc.date.issued2024-01-15en_AU
dc.date.statistics2023-12-10en_AU
dc.description.abstractHypothesis Nanomaterials have been extensively investigated for a wide range of biomedical applications, including as antimicrobial agents, drug delivery vehicles, and diagnostic devices. The commonality between these biomedical applications is the necessity for the nanoparticle to interact with or pass through the cellular wall and membrane. Cell-nanomaterial interactions/uptake can occur in various ways, including adhering to the cell wall, forming aggregates on the surface, becoming absorbed within the cell wall itself, or transversing into the cell cytoplasm. These interactions are common to mammalian cells, bacteria, and yeast cells. This variety of interactions can cause changes to the integrity of the cell wall and the cell overall, but the precise mechanisms underpinning such interactions remain poorly understood. Here, we investigate the interaction between commonly investigated gold nanoparticles (AuNPs) and the cell wall/membrane of a model fungal cell to explore the general effects of interaction and uptake. Experiments The interactions between 100 nm citrate-capped AuNPs and the cell wall of Candida albicans fungal cells were studied using a range of advanced microscopy techniques, including atomic force microscopy, confocal laser scanning microscopy, scanning electron microscopy, transmission electron microscopy, and synchrotron-FTIR micro-spectroscopy. Findings In most cases, particles adhered on the cell surface, although instances of particles being up-taken into the cell cytoplasm and localised within the cell wall and membrane were also observed. There was a measurable increase in the stiffness of the fungal cell after AuNPs were introduced. Analysis of the synchrotron-FTIR data showed significant changes in spectral features associated with phospholipids and proteins after exposure to AuNPs. © 2023 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license.en_AU
dc.description.sponsorshipZ.L.S. acknowledges this research was supported by an AINSE Ltd. Postgraduate Research Award (PGRA: ALNSTU13134). A.E. is supported by an Australian Research Council (ARC) Discovery Early Career Research Award (DECRA) (DE220100511). The authors would like to acknowledge and thank both the Microscopy and Microanalysis Facility (RMMF) and the MicroNano Research Facility (MNRF) at RMIT University for the use of their facilities and the expertise of their staff.en_AU
dc.identifier.citationPenman, R., Kariuki, R., Shaw, Z. L., Dekiwadia, C., Christofferson, A. J., Bryant, G., Vongsvivut, J., Bryant, S. J., & Elbourne, A. (2024). Gold nanoparticle adsorption alters the cell stiffness and cell wall bio-chemical landscape of Candida albicans fungal cells. Journal of Colloid and Interface Science, 654, Part A, 390-404. doi:10.1016/j.jcis.2023.10.017en_AU
dc.identifier.issn0021-9797en_AU
dc.identifier.issuePart Aen_AU
dc.identifier.journaltitleJournal of Colloid and Interface Scienceen_AU
dc.identifier.pagination390-404en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15337en_AU
dc.identifier.volume654en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.relation.urihttps://doi.org/10.1016/j.jcis.2023.10.017en_AU
dc.subjectFungien_AU
dc.subjectNanoparticlesen_AU
dc.subjectAtomic force microscopyen_AU
dc.subjectSynchrotronsen_AU
dc.subjectAttenuationen_AU
dc.subjectReflectionen_AU
dc.subjectGolden_AU
dc.subjectAdsorptionen_AU
dc.subjectCell wallen_AU
dc.titleGold nanoparticle adsorption alters the cell stiffness and cell wall bio-chemical landscape of Candida albicans fungal cellsen_AU
dc.typeJournal Articleen_AU
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