Antimicrobial and anti-Inflammatory gallium Implanted ‘trojan Horse’ surfaces for implantable devices

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dc.contributor.authorDivakarla, SKen_AU
dc.contributor.authorDas, Ten_AU
dc.contributor.authorChatterjee, Cen_AU
dc.contributor.authorIonescu, Men_AU
dc.contributor.authorPastuovic, Zen_AU
dc.contributor.authorJang, JHen_AU
dc.contributor.authorAlkhoury, Hen_AU
dc.contributor.authorLoppnow, Hen_AU
dc.contributor.authorYamaguchi, Sen_AU
dc.contributor.authorGroth, Ten_AU
dc.contributor.authorChrzanowski, Wen_AU
dc.date.accessioned2024-02-28T04:26:47Zen_AU
dc.date.available2024-02-28T04:26:47Zen_AU
dc.date.issued2021-11-23en_AU
dc.date.statistics2023-04-26en_AU
dc.description.abstractA rapidly aging population, high incidence of osteoporosis and trauma-related fractures, and better health care access explain rapid surge in utilisation of orthopedic implantable devices. Unfortunately, many implants fail without strategies that synergistically prevent infections and enhance the implant’s integration with host tissues. Here, we propose a solution that builds on our pioneering work on gallium (Ga)-enhanced biomaterials, which show exceptional antimicrobial activity, and combined it with defensin (De, hBD-1), which has potent anti-microbial activity in vivo as part of the innate immune system. Our aim was to simultaneously impart antimicrobial activity and anti-inflammatory properties to polymer-based implantable devices through the modification of the surfaces with Ga ions and immobilisation of De. Poly-lactic acid (PLA) films were modified using Ga implantation using the Surface Engineering Beamline of the 6MV SIRIUS tandem accelerator at ANSTO Australia, and subsequently functionalised with De. Ga ion implantation increased surface roughness and increased stiffness of treated PLA surfaces and led to the reduction in foreign body giant cell formation and expression of pro-inflammatory cytokine IL-1β. Ga implantation and defensin immobilization both independently and synergistically introduced antimicrobial activity to the surfaces, significantly reducing total live biomass. We demonstrated, for the first time, that antimicrobial effects of De were enhanced by its surface immobilization. Cumulatively, the Ga-De surfaces were able to kill bacteria and reduce inflammation in comparison to the untreated control. These innovative surfaces have the potential to prevent biofilm formation without inducing cellular toxicity or inflammation, which is essential in enhancing integration of implantable devices with host tissues and hence, ensure their longevity. © The Authorsen_AU
dc.identifier.citationDivakarla, S. K. Das, T., Chatterjee, C., Isonesu, M., Pastuovic, Z., Jang, J.-H., Alkhoury, H., Loppnow, H., Yamaguchi, S., Goth, T., & Chrzanowski, W., (2021). Antimicrobial and anti-Inflammatory gallium Implanted ‘trojan Horse’ surfaces for implantable devices. Presentation to the ANSTO User Meeting 2021, 24-26 November 2021, Online. Retrieved from: https://events01.synchrotron.org.au/event/146/contributions/4269/en_AU
dc.identifier.conferenceenddate2021-11-26en_AU
dc.identifier.conferencenameANSTO User Meeting 2021en_AU
dc.identifier.conferenceplaceOnlineen_AU
dc.identifier.conferencestartdate2021-11-24en_AU
dc.identifier.urihttps://events01.synchrotron.org.au/event/146/contributions/4269/en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15474en_AU
dc.language.isoenen_AU
dc.publisherAustralian Nuclear Science and Technology Organisationen_AU
dc.subjectAntimicrobial agentsen_AU
dc.subjectAntipyreticsen_AU
dc.subjectGalliumen_AU
dc.subjectImplantsen_AU
dc.subjectANSTOen_AU
dc.subjectAustralian organizationsen_AU
dc.subjectPolymersen_AU
dc.subjectAcceleratorsen_AU
dc.titleAntimicrobial and anti-Inflammatory gallium Implanted ‘trojan Horse’ surfaces for implantable devicesen_AU
dc.typeConference Presentationen_AU
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