Synchrotron x-ray fluorescence microscopy-enabled elemental mapping illuminates the ‘battle for nutrients’ between plant and pathogen

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dc.contributor.authorNaim, Fen_AU
dc.contributor.authorKhambatta, Ken_AU
dc.contributor.authorSanglard, LMVPen_AU
dc.contributor.authorSauzier, Gen_AU
dc.contributor.authorReinhardt, Jen_AU
dc.contributor.authorPaterson, DJen_AU
dc.contributor.authorZerihun, Aen_AU
dc.contributor.authorHackett, MJen_AU
dc.contributor.authorGibberd, MRen_AU
dc.date.accessioned2024-11-15T02:25:23Zen_AU
dc.date.available2024-11-15T02:25:23Zen_AU
dc.date.issued2021-03-29en_AU
dc.date.statistics2024-11-08en_AU
dc.description.abstractMetal homeostasis is integral to normal plant growth and development. During plant–pathogen interactions, the host and pathogen compete for the same nutrients, potentially impacting nutritional homeostasis. Our knowledge of outcome of the interaction in terms of metal homeostasis is still limited. Here, we employed the X-ray fluorescence microscopy (XFM) beamline at the Australian Synchrotron to visualize and analyse the fate of nutrients in wheat leaves infected with Pyrenophora tritici-repentis, a necrotrophic fungal pathogen. We sought to (i) evaluate the utility of XFM for sub-micron mapping of essential mineral nutrients and (ii) examine the spatiotemporal impact of a pathogen on nutrient distribution in leaves. XFM maps of K, Ca, Fe, Cu, Mn, and Zn revealed substantial hyperaccumulation within, and depletion around, the infected region relative to uninfected control samples. Fungal mycelia were visualized as thread-like structures in the Cu and Zn maps. The hyperaccumulation of Mn in the lesion and localized depletion in asymptomatic tissue surrounding the lesion was unexpected. Similarly, Ca accumulated at the periphery of the symptomatic region and as microaccumulations aligning with fungal mycelia. Collectively, our results highlight that XFM imaging provides the capability for high-resolution mapping of elements to probe nutrient distribution in hydrated diseased leaves in situ. © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.en_AU
dc.description.sponsorshipThis research to generate elemental maps and their analysis was undertaken on the X-ray Fluorescence Microscopy beamline at the Australian Synchrotron, part of Australia’s Nuclear Science and Technology Organisation (ANSTO). This work was generously supported by grants from the Australian Grains Research and Development Corporation (GRDC), Curtin University, and ANSTO. The authors thank Dr Pao Theen See, Ms Elzette Wentzel, and Mr John Jackson for their time and guidance in laboratory preparation of fungal cultures, inoculation of wheat leaves, and staining of fungal mycelia.en_AU
dc.format.mediumPrinten_AU
dc.identifier.citationNaim, F., Khambatta, K., Sanglard, L. M. V. P., Sauzier, G., Reinhardt, J., Paterson, D. J., Zerihun, A., Hackett, M. J., & Gibberd, M. R. (2021). Synchrotron x-ray fluorescence microscopy-enabled elemental mapping illuminates the ‘battle for nutrients’ between plant and pathogen. Journal of Experimental Botany, 72(7), 2757-2768. doi:10.1093/jxb/erab005en_AU
dc.identifier.issn0022-0957en_AU
dc.identifier.issn1460-2431en_AU
dc.identifier.issue7en_AU
dc.identifier.journaltitleJournal of Experimental Botanyen_AU
dc.identifier.pagination2757-2768en_AU
dc.identifier.urihttps://doi.org/10.1093/jxb/erab005en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15749en_AU
dc.identifier.volume72en_AU
dc.languageEnglishen_AU
dc.language.isoenen_AU
dc.publisherOxford University Pressen_AU
dc.subjectMicroscopyen_AU
dc.subjectSynchrotronsen_AU
dc.subjectFluorescenceen_AU
dc.subjectNutrientsen_AU
dc.subjectPlantsen_AU
dc.subjectPathogensen_AU
dc.subjectMetalsen_AU
dc.subjectHomeostasisen_AU
dc.subjectGrowthen_AU
dc.subjectFungien_AU
dc.titleSynchrotron x-ray fluorescence microscopy-enabled elemental mapping illuminates the ‘battle for nutrients’ between plant and pathogenen_AU
dc.typeJournal Articleen_AU
dcterms.dateAccepted2021-01-13en_AU
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