Annexin V-containing cubosomes for targeted early detection of apoptosis in degenerative retinal tissue

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dc.contributor.authorDing, Yen_AU
dc.contributor.authorChow, SHen_AU
dc.contributor.authorLiu, GSen_AU
dc.contributor.authorWang, Ben_AU
dc.contributor.authorLin, TWen_AU
dc.contributor.authorHsu, HYen_AU
dc.contributor.authorDuff, APen_AU
dc.contributor.authorLe Brun, APen_AU
dc.contributor.authorShen, HHen_AU
dc.date.accessioned2021-01-11T01:34:00Zen_AU
dc.date.available2021-01-11T01:34:00Zen_AU
dc.date.issued2018-10-26en_AU
dc.date.statistics2021-01-08en_AU
dc.description.abstractNew drug delivery materials targeting damaged ocular tissues are of particular interest. In this work, we have formulated annexin/phosphatidylserine/phytantriol and annexin/phosphatidylserine/monoolein cubosomes based on incorporation of 1,2-dipalmitoyl-sn-glycero-3-phospho-L-serine (PS) lipid and annexin V (ANX) protein with phytantriol (Phy) and monoolein (MO) respectively. The incorporation of ANX is important because it can be used as a diagnostic tool for in vivo apoptosis detection due to its high affinity to phosphatidylserine in the presence of Ca2+. We have also prepared PS–Phy and PS–MO cubosomes without ANX as a comparison, and characterized them using dynamic light scattering, cryo-TEM images and small-angle X-ray scattering, showing that PS–Phy cubosomes have greater chemical stability, and that ANX–PS–Phy cubosomes have the potential for in vivo drug delivery. In addition, we have reconstituted an apoptotic biomimetic membrane on a surface to gain insights into cubosome–bilayer interactions using a quartz-crystal microbalance and neutron reflectometry. The neutron reflectivity data reveal that there is exchange of materials between the biomimetic apoptotic bilayer and ANX–PS–Phy cubosomes, with an accumulation of ANX between the membrane and cubosomes possibly being the reason for the reduced cytotoxicity of ANX–PS–Phy cubosomes. A rat model of laser-induced choroidal neovascularization showed that ANX–PS–Phy cubosomes specifically targeted apoptotic cells in vivo. We propose that ANX–PS–Phy cubosomes are a potential candidate for ocular drug delivery for eye diseases. © The Royal Society of Chemistry 2018en_AU
dc.identifier.citationDing, Y., Chow, S. H., Liu, G.-S., Wang, B., Lin, T.-W., Hsu, H.-Y., Duff, A. P. Le Brun, A. P., & Shen, H.-H. (2018). Annexin V-containing cubosomes for targeted early detection of apoptosis in degenerative retinal tissue. Journal of Materials Chemistry B, 6(46), 7652-7661. doi:10.1039/c8tb02465ken_AU
dc.identifier.issn2050-7518en_AU
dc.identifier.issue46en_AU
dc.identifier.journaltitleJournal of Materials Chemistry Ben_AU
dc.identifier.pagination7652-7661en_AU
dc.identifier.urihttps://doi.org/10.1039/c8tb02465ken_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/10231en_AU
dc.identifier.volume6en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectAntibioticsen_AU
dc.subjectPhotosynthesisen_AU
dc.subjectCell killingen_AU
dc.subjectDrug deliveryen_AU
dc.subjectLight scatteringen_AU
dc.subjectOphthalmologyen_AU
dc.subjectReflectionen_AU
dc.subjectX-ray spectroscopyen_AU
dc.titleAnnexin V-containing cubosomes for targeted early detection of apoptosis in degenerative retinal tissueen_AU
dc.typeJournal Articleen_AU
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