Synthesis of perdeuterated 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine ([D82]POPC) for neutron studies on bilayer lipid membranes

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dc.contributor.authorYepuri, NRen_AU
dc.contributor.authorDarwish, TAen_AU
dc.contributor.authorLeung, Aen_AU
dc.contributor.authorKrause-Heuer, AMen_AU
dc.contributor.authorWacklin, HPen_AU
dc.contributor.authorDelhom, Ren_AU
dc.contributor.authorHolden, PJen_AU
dc.date.accessioned2021-08-24T21:14:43Zen_AU
dc.date.available2021-08-24T21:14:43Zen_AU
dc.date.issued2017-07-12en_AU
dc.date.statistics2021-08-16en_AU
dc.description.abstractThe complexity of the chemical synthesis of completely deuterium-labelled unsaturated lipids has meant that most neutron experiments, to date, have been restricted to saturated phospholipid species, the behaviour of which under physiological conditions may not be representative of the unsaturated varieties found in biological membranes. Unsaturated lipids occur widely in nature and are crucial for the fluidity of cell membranes. Biologically relevant phospholipids such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) are typically asymmetric and cis-9 unsaturated in the sn-2-acyl chain, where as the sn-1 chain is often saturated. Commercially available sn-1 chain deuterated [D31]1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine([D31]POPC) does not provide enough contrast for detailed structural investigation through neutron studies, and is nearly perfectly contrast-matched to most proteins and membrane binding peptides in heavy water, which makes them neutron invisible. With this background in mind, we have developed a method for the synthesis of perdeuterated POPC and its partially labelled form.[1] The structure of a supported bilayer membrane formed from these lipids was determined by measuring neutron reflectivity in a series of solvent contrasts, which will be discussed in this paper.en_AU
dc.identifier.citationYepuri, N. R., Darwish, T., Leung, A., Krause‐Heuer, A., Wacklin, H., Delhom, R., & Holden, P. (2017). Synthesis of perdeuterated 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine ([D82]POPC) for neutron studies on bilayer lipid membranes. Paper presented at ICNS 2017 (International Conference on Neutron Scattering), Daejeon, South Korea, 9 to 13 July 2017. Retrieved from: http://www.icns2017.org/program.phpen_AU
dc.identifier.conferenceenddate13 July 2017en_AU
dc.identifier.conferencenameICNS 2017 (International Conference on Neutron Scattering)en_AU
dc.identifier.conferenceplaceDaejeon, South Koreaen_AU
dc.identifier.conferencestartdate9 July 2017en_AU
dc.identifier.urihttp://www.icns2017.org/program.phpen_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/11459en_AU
dc.language.isoenen_AU
dc.publisherInternational Conference on Neutron Scatteringen_AU
dc.subjectDeuteriumen_AU
dc.subjectLipidsen_AU
dc.subjectPhospholipidsen_AU
dc.subjectPeptidesen_AU
dc.subjectHeavy wateren_AU
dc.subjectNeutron reflectorsen_AU
dc.titleSynthesis of perdeuterated 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine ([D82]POPC) for neutron studies on bilayer lipid membranesen_AU
dc.typeConference Abstracten_AU
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