Synchrotron CT of an equine digit at the Australian Synchrotron imaging and medical beamline

Simple item page
dc.contributor.authorMontgomery, JBen_AU
dc.contributor.authorKlein, Men_AU
dc.contributor.authorBoire, JRen_AU
dc.contributor.authorBeck, Cen_AU
dc.contributor.authorHäusermann, Den_AU
dc.contributor.authorMaksimenko, Aen_AU
dc.contributor.authorHall, CJen_AU
dc.date.accessioned2025-03-21T03:24:53Zen_AU
dc.date.available2025-03-21T03:24:53Zen_AU
dc.date.issued2021-11en_AU
dc.date.statistics2025-03-12en_AU
dc.description.abstractLaminitis is an extremely painful and debilitating condition of horses that can affect their athletic ability and even quality of life. The current gold standard for assessment of laminar tissue is histology, which is the only modality that enables detailed visualization of the lamina. Histology requires dissection of the hoof and therefore can only represent one specific time point. The superior spatial and contrast resolution of synchrotron computed tomography (sCT), when compared with readily available imaging modalities, such as radiographs and conventional CT, provides an opportunity for detailed studies of the lamina without the need for hoof dissection and histological assessment. If the resolution of histology can be matched or even approached, dynamic events, such as laminar blood flow, could also be studied on the microscopic tissue level. To investigate this possible application of sCT further, two objectives are presented: (i) to develop a protocol for sCT of an equine digit using cadaver limbs and (ii) to apply the imaging protocol established during (i) for sCT imaging of the vasculature within the foot using an ex vivo perfusion system to deliver the vascular contrast. The hypotheses were that sCT would allow sufficient resolution for detailed visualization to the level of the secondary lamellae and associated capillaries within the equine digit. Synchrotron CT enabled good visualization of the primary lamellae (average length 3.6 mm) and the ex vivo perfusion system was able to deliver vascular contrast agent to the vessels of the lamina. The individual secondary lamellae (average length 0.142 mm) could not be seen in detail, although differentiation between primary and secondary lamellae was achieved. This approaches, but does not yet reach, the current gold standard, histology, for assessment of the lamellae; however, with further refinement of this imaging technique, improved resolution may be accomplished in future studies. © 2021 The Authors - Open Access CC BY.en_AU
dc.description.sponsorshipThis study was performed at the Australian Synchrotron (ANSTO) Imaging and Medical Beamline (proposal number 15938). We would like to acknowledge Dr Travis Smyth from Ballarat Equine Veterinary Practice for providing us with equine cadaver limbs, including those obtained from the horse with laminitis. Ballarat Equine Veterinary Practice further supplied the radiographs, shared here with their permission. We further would like to acknowledge Dr Andrew Stent, Head of Anatomic Pathology Diagnostic Service from the University of Melbourne.en_AU
dc.format.mediumPrint-Electronicen_AU
dc.identifier.citationMontgomery, J. B., Klein, M., Boire, J. R., Beck, C., Hausermann, D., Maksimenko, A., & Hall, C. J. (2021). Synchrotron CT of an equine digit at the Australian Synchrotron imaging and medical beamline. Journal of Synchrotron Radiation, 28(6), 1769-1777. doi:10.1107/S1600577521010493en_AU
dc.identifier.issn0909-0495en_AU
dc.identifier.issn1600-5775en_AU
dc.identifier.issue6en_AU
dc.identifier.journaltitleJournal of Synchrotron Radiationen_AU
dc.identifier.pagination1769-1777en_AU
dc.identifier.urihttps://doi.org/10.1107/s1600577521010493en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/16084en_AU
dc.identifier.volume28en_AU
dc.languageengen_AU
dc.language.isoenen_AU
dc.publisherInternational Union of Crystallographyen_AU
dc.subjectSynchrotronsen_AU
dc.subjectAustraliaen_AU
dc.subjectHorsesen_AU
dc.subjectHistologyen_AU
dc.subjectMedicineen_AU
dc.subjectBlood flowen_AU
dc.subjectLaminar flowen_AU
dc.subjectNMR imagingen_AU
dc.subjectFeeten_AU
dc.titleSynchrotron CT of an equine digit at the Australian Synchrotron imaging and medical beamlineen_AU
dc.typeJournal Articleen_AU
dcterms.dateAccepted2021-10-10en_AU
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Synchrotron CT of an equine digit at the Australian Synchrotron Imaging and Medical Beamline.pdf
Size:
2.18 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download
Thumbnail Image
Name:
tv5028.pdf
Size:
2.18 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.66 KB
Format:
Plain Text
Description:
Download
Collections
Journal Articles