Using humidity to control the morphology and properties of electrospun bioPEGylated polyhydroxybutyrate scaffolds

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dc.contributor.authorFoster, LJRen_AU
dc.contributor.authorChan, RTHen_AU
dc.contributor.authorRussell, RAen_AU
dc.contributor.authorHolden, PJen_AU
dc.date.accessioned2024-02-27T01:17:33Zen_AU
dc.date.available2024-02-27T01:17:33Zen_AU
dc.date.issued2020-10-05en_AU
dc.date.statistics2024-02-27en_AU
dc.description.abstractElectrospinning produces nanofibrous scaffolds with potential for tissue engineering and wound repair. Spinning parameters control scaffold morphology and properties. BioPEGylation of polyhydroxybutyrate (PHB) introduces terminal hydrophilic groups into the hydrophobic chain, making this natural-synthetic hybrid copolymer more susceptible to humidity. Varying the humidity from 10 to 50% RH during electrospinning had a relatively little effect on polyhydroxybutyrate (PHB) average fiber and pore diameters, which remained around 3.0 and 8.7 μm, respectively. In contrast, fiber and pore diameters for electrospun bioPEGylated PHB scaffolds varied significantly with humidity, peaking at 30% RH (5.5 and 14.1 μm, respectively). While scaffolds showed little change, hydrophobicity decreased linearly with humidity during electrospinning. Compared to solvent-cast films, electrospun scaffolds showed significantly greater average cell spread. A 108% increase for olfactory ensheathing cells (OECs) cultivated on bioPEGylated PHB scaffolds was proportionally greater than their counterparts on electrospun PHB scaffolds, (70%). OECS grown on bioPEGylated PHB scaffolds were over twice the size, 260 ± 20 μm diameter, than those on PHB electrospun scaffolds, 110 ± 18 μm diameter. Electrospun scaffolds also promoted cell health compared to their solvent-cast counterparts, with increases in the mitochondrial activity of 165 ± 13 and 196 ± 13% for PHB and bioPEGylated PHB, respectively. OECS cultivated on electrospun scaffolds of bioPEGylated PHB had significantly better membrane integrities compared to their counterparts on solvent-cast films, 47 ± 5% reducing to 17 ± 6%. The combination of bioPEGylation and humidity during electrospinning permitted significant controllable changes to scaffold morphology and properties. These changes resulted in the significantly greater promotion of cell growth on electrospun bioPEGylated PHB scaffolds compared to their solvent-cast counterparts and electrospun PHB. © 2020 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.en_AU
dc.description.sponsorshipThe authors acknowledge the funding and support of the Australian Nuclear Science Organization (ANSTO) and a University of New South Wales (UNSW) Goldstar Award to L.J.R.F.en_AU
dc.format.mediumElectronic-eCollectionen_AU
dc.identifier.citationFoster, L. J. R., Chan, R. T. H.,, Russell, R. A., & Holden, P. J. (2020). Using humidity to control the morphology and properties of electrospun bioPEGylated polyhydroxybutyrate scaffolds. ACS omega, 5(41), 26476-26485. doi:10.1021/acsomega.0c02993en_AU
dc.identifier.issn2470-1343en_AU
dc.identifier.issue41en_AU
dc.identifier.journaltitleACS Omegaen_AU
dc.identifier.pagination26476-26485en_AU
dc.identifier.urihttp://dx.doi.org/10.1021/acsomega.0c02993en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15442en_AU
dc.identifier.volume5en_AU
dc.languageEnglishen_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Society (ACS)en_AU
dc.subjectSpinen_AU
dc.subjectFibersen_AU
dc.subjectHumidityen_AU
dc.subjectPolymersen_AU
dc.subjectMorphologyen_AU
dc.subjectCopolymersen_AU
dc.titleUsing humidity to control the morphology and properties of electrospun bioPEGylated polyhydroxybutyrate scaffoldsen_AU
dc.typeJournal Articleen_AU
dcterms.dateAccepted2020-09-23en_AU
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