Determining glucose metabolism kineticsuUsing 18F-FDG micro-PET/CT

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dc.contributor.authorCochran, BJen_AU
dc.contributor.authorRyder, WJen_AU
dc.contributor.authorParmar, Aen_AU
dc.contributor.authorKlaeser, Ken_AU
dc.contributor.authorReilhac, Aen_AU
dc.contributor.authorAngelis, GIen_AU
dc.contributor.authorMeikle, SRen_AU
dc.contributor.authorBarter, PJen_AU
dc.contributor.authorRye, KAen_AU
dc.date.accessioned2023-11-27T00:35:36Zen_AU
dc.date.available2023-11-27T00:35:36Zen_AU
dc.date.issued2017-05-02en_AU
dc.date.statistics2022-05-24en_AU
dc.description.abstractThis paper describes the use of 18F-FDG and micro-PET/CT imaging to determine in vivo glucose metabolism kinetics in mice (and is transferable to rats). Impaired uptake and metabolism of glucose in multiple organ systems due to insulin resistance is a hallmark of type 2 diabetes. The ability of this technique to extract an image-derived input function from the vena cava using an iterative deconvolution method eliminates the requirement of the collection of arterial blood samples. Fitting of tissue and vena cava time activity curves to a two-tissue, three compartment model permits the estimation of kinetic micro-parameters related to the 18F-FDG uptake from the plasma to the intracellular space, the rate of transport from intracellular space to plasma and the rate of 18F-FDG phosphorylation. This methodology allows for multiple measures of glucose uptake and metabolism kinetics in the context of longitudinal studies and also provides insights into the efficacy of therapeutic interventions. © 2022 MyJoVE Corporationen_AU
dc.description.sponsorshipThis work was supported by a National Imaging Facility Subsidised Access Grant to BJC, a National Health and Medical Research Council of Australia program grant (482800) to KAR and PJB. The authors would like to thank Andrew Arthur, Hasar Hazme and Marie-Claude Gregoire for support in developing this method.en_AU
dc.identifier.articlenumbere55184en_AU
dc.identifier.citationCochran, B. J., Ryder, W. J., Parmar, A., Klaeser, K., Reilhac, A., Angelis, G. I., Meikle, S. R., Barter, P. J., & Rye, K.-A. (2017). Determining glucose metabolism kineticsuUsing 18F-FDG micro-PET/CT, JoVE: Journal of Visualized Experiments (123), e55184. doi:10.3791/55184en_AU
dc.identifier.issn1940-087Xen_AU
dc.identifier.journaltitleJoVE: Journal of Visualized Experimentsen_AU
dc.identifier.urihttps://doi.org/doi:10.3791/55184en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15243en_AU
dc.identifier.volume123en_AU
dc.language.isoenen_AU
dc.publisherMyJoVE Corporation.en_AU
dc.relation.urihttps://doi.org/doi:10.3791/55184en_AU
dc.subjectGlucoseen_AU
dc.subjectMetabolismen_AU
dc.subjectFluorine 18en_AU
dc.subjectFluorine isotopesen_AU
dc.subjectPositron computed tomographyen_AU
dc.subjectDiabetes mellitusen_AU
dc.subjectRatsen_AU
dc.subjectPhosphorylationen_AU
dc.subjectCAT scanningen_AU
dc.titleDetermining glucose metabolism kineticsuUsing 18F-FDG micro-PET/CTen_AU
dc.typeJournal Articleen_AU
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