Turn-on fluorescence aptasensor on magnetic nanobeads for aflatoxin M1 detection based on an exonuclease III-assisted signal amplification strategy

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dc.contributor.authorZhang, FYen_AU
dc.contributor.authorLiu, LYen_AU
dc.contributor.authorNi, SNen_AU
dc.contributor.authorDeng, JKen_AU
dc.contributor.authorLiu, GJen_AU
dc.contributor.authorMiddleton, RJen_AU
dc.contributor.authorInglis, DWen_AU
dc.contributor.authorWang, Sen_AU
dc.contributor.authorLiu, GZen_AU
dc.date.accessioned2022-08-29T02:02:03Zen_AU
dc.date.available2022-08-29T02:02:03Zen_AU
dc.date.issued2019-01-16en_AU
dc.date.statistics2022-08-04en_AU
dc.descriptionThis is an open access article distributed under the Creative Commons Attribution License.en_AU
dc.description.abstractIn order to satisfy the need for sensitive detection of Aflatoxin M1 (AFM1), we constructed a simple and signal-on fluorescence aptasensor based on an autocatalytic Exonuclease III (Exo III)-assisted signal amplification strategy. In this sensor, the DNA hybridization on magnetic nanobeads could be triggered by the target AFM1, resulting in the release of a single-stranded DNA to induce an Exo III-assisted signal amplification, in which numerous G-quadruplex structures would be produced and then associated with the fluorescent dye to generate significantly amplified fluorescence signals resulting in the increased sensitivity. Under the optimized conditions, this aptasensor was able to detect AFM1 with a practical detection limit of 9.73 ng kg−1 in milk samples. Furthermore, the prepared sensor was successfully used for detection of AFM1 in the commercially available milk samples with the recovery percentages ranging from 80.13% to 108.67%. Also, the sensor performance was evaluated by the commercial immunoassay kit with satisfactory results. © 2019 the Authorsen_AU
dc.description.sponsorshipThis work was financially supported by funding from the ARC Future Fellowship (FT160100039), the National Natural Science Foundation of China (Grant 21575045) and the ARC Centre of Excellence for Nanoscale BioPhotonics (CE140100003). F.Z. was supported by the Macquarie University Cotutelle-iMQRTP scholarship (2017501) and Postgraduate Research Scholarship (PGRA) funded by Australian Institute of Nuclear Science and Engineering (AINSE).en_AU
dc.identifier.citationZhang, F., Liu, L., Ni, S., Deng, J., Liu, G.-J., Middleton, R., Inglis, D. W., Wang, S., & Liu, G. (2019). Turn-on fluorescence aptasensor on magnetic nanobeads for aflatoxin M1 detection based on an exonuclease III-assisted signal amplification strategy. Nanomaterials, 9(1), 104. doi:10.3390/nano9010104en_AU
dc.identifier.issn2079-4991en_AU
dc.identifier.issue4en_AU
dc.identifier.journaltitleNanomaterialsen_AU
dc.identifier.pagination104en_AU
dc.identifier.urihttps://doi.org/10.3390/nano9010104en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/13640en_AU
dc.identifier.volume9en_AU
dc.language.isoenen_AU
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)en_AU
dc.subjectFluorescenceen_AU
dc.subjectAflatoxinsen_AU
dc.subjectNanotechnologyen_AU
dc.subjectHybridizationen_AU
dc.subjectDetectionen_AU
dc.subjectTargetsen_AU
dc.titleTurn-on fluorescence aptasensor on magnetic nanobeads for aflatoxin M1 detection based on an exonuclease III-assisted signal amplification strategyen_AU
dc.typeJournal Articleen_AU
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