Deuteration for biological SANS: case studies, success and challenges in chemistry and biology

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dc.contributor.authorDuff, APen_AU
dc.contributor.authorCagnes, MPen_AU
dc.contributor.authorDarwish, TAen_AU
dc.contributor.authorKrause-Heuer, AMen_AU
dc.contributor.authorMoir, Men_AU
dc.contributor.authorRecsei, Cen_AU
dc.contributor.authorRekas, Aen_AU
dc.contributor.authorRussell, RAen_AU
dc.contributor.authorWilde, KLen_AU
dc.contributor.authorYepuri, NRen_AU
dc.date.accessioned2024-03-01T01:15:56Zen_AU
dc.date.available2024-03-01T01:15:56Zen_AU
dc.date.issued2022-11en_AU
dc.date.statistics2024-02-23en_AU
dc.description.abstractSmall angle neutron scattering is a powerful complementary technique in structural biology. It generally requires, or benefits from, deuteration to achieve its unique potentials. Molecular deuteration has become a mature expertise, with deuteration facilities located worldwide to support access to the technique for a wide breadth of structural biology and life sciences. The sorts of problems well answered by small angle scattering and deuteration involve large (> 10 Å) scale flexible movements, and this approach is best used where high-resolution methods (crystallography, NMR, cryo-EM) leave questions unanswered. This chapter introduces deuteration, reviewing biological deuteration of proteins, lipids and sterols, and then steps through the ever-expanding range of deuterated molecules being produced by chemical synthesis and enabling sophisticated experiments using physiologically relevant lipids. Case studies of recent successful use of deuteration may provide illustrative examples for strategies for future experiments. We discuss issues of nomenclature for synthesised molecules of novel labeling and make recommendations for their naming. We reflect on our experiences, with cost associated with achieving an arbitrary deuteration level, and on the benefits of experimental co-design by user scientist, deuteration scientist, and neutron scattering scientist working together. Although methods for biological and chemical deuteration are published in the public domain, we recommend that the best method to deuterate is to engage with a deuteration facility. © 2022 Elsevieren_AU
dc.identifier.booktitleSmall angle scattering Part A: Methods for structural investigationen_AU
dc.identifier.chapter3en_AU
dc.identifier.citationDuff, A. P., Cagnes, M., Darwish, T. A., Krause-Heuer, A. M., Moir, M., Recsei, C., Rekas, A., Russell, R. A., Wilde, K. L., & Yepuri, N. R. (2022). Deuteration for biological SANS: Case studies, success and challenges in chemistry and biology. In J. A. Tainer (Ed), Small Angle Scattering Part A: Methods for Structural Investigation, (pp. 85-126), Methods in Enzymology (Vol. 677). Cambridge, Massachusetts: Academic Press. doi:10.1016/bs.mie.2022.08.030en_AU
dc.identifier.edition1sten_AU
dc.identifier.editorsJ. A. Taineren_AU
dc.identifier.isbn9780323991803en_AU
dc.identifier.issn0076-6879en_AU
dc.identifier.pagination85-126en_AU
dc.identifier.placeofpublicationCambridge, Massachusettsen_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15518en_AU
dc.identifier.volume677en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.relation.ispartofseriesMethods in enzymology; 677en_AU
dc.relation.urihttp://dx.doi.org/10.1016/bs.mie.2022.08.030en_AU
dc.subjectDeuterationen_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectChemistryen_AU
dc.subjectBiologyen_AU
dc.subjectCrystallographyen_AU
dc.subjectNMR imagingen_AU
dc.subjectProteinsen_AU
dc.subjectLipidsen_AU
dc.titleDeuteration for biological SANS: case studies, success and challenges in chemistry and biologyen_AU
dc.typeBook chapteren_AU
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