Browsing by Author "Fan, XW"

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    A technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiology
    (Springer Nature, 2022-07-09) Fan, XW; Lee, KM; Jones, MWM; Howard, DL; Crawford, R; Prasadam, I
    The anatomy of the osteochondral junction is complex because several tissue components exist as a unit, including uncalcified cartilage (with superficial, middle, and deep layers), calcified cartilage, and subchondral bone. Furthermore, it is difficult to study because this region is made up of a variety of cell types and extracellular matrix compositions. Using X-ray fluorescence microscopy, we present a protocol for simultaneous elemental detection on fresh frozen samples. We transferred the osteochondral sample using a tape-assisted system and successfully tested it in synchrotron X-ray fluorescence. This protocol elucidates the distinct distribution of elements at the human knee’s osteochondral junction, making it a useful tool for analyzing the co-distribution of various elements in both healthy and diseased states. © The Author(s) 2022 - Open Access under a Creative Commons Attribution 4.0 International License,