Fully automated one-pot radiosynthesis of O-(2-[18F]fluoroethyl)-L-tyrosine on the TracerLab FXFN module
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Date
2011-07-01
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Publisher
Elsevier B.V.
Abstract
Introduction: An efficient fully automated method for the radiosynthesis of enantiomerically pure O-(2-[18F]fluoroethyl)-L-tyrosine ([18F]FET) using the GE TracerLab FXFN synthesis module via the O-(2-tosyloxyethyl)-N-trityl-L-tyrosine tert-butylester precursor has been developed. Methods: The radiolabelling of [18F]FET involved a classical [18F]fluoride nucleophilic substitution performed in acetonitrile using potassium carbonate and Kryptofix 222, followed by acid hydrolysis using 2N hydrochloric acid. Results: [18F]FET was produced in 35±5% (n=22) yield non-decay-corrected (55±5% decay-corrected) and with radiochemical and enantiomeric purity of >99% with a specific activity of >90 GBq/μmol after 63 min of radiosynthesis including HPLC purification and formulation. Conclusion: The automated radiosynthesis provides high and reproducible yields suitable for routine clinical use. © 2011 Elsevier Inc.
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Keywords
Amino acids, Fluorination, Tracer techniques, Synthesis, Positron computed tomography, Neoplasms, Brain, Pharmacology
Citation
Bourdier, T., Greguric, I., Roselt, P., Jackson, T., Faragalla, J., & Katsifis, A. (2011). Fully automated one-pot radiosynthesis of O-(2-[18 F] fluoroethyl)-l-tyrosine on the TracerLab FX FN module. Nuclear Medicine and Biology, 38(5), 645-651. doi:10.1016/j.nucmedbio.2011.01.001