ANSTO Publications Online >
Journal Publications >
Journal Articles >

Please use this identifier to cite or link to this item: http://apo.ansto.gov.au/dspace/handle/10238/8147

Title: Small-angle scattering as a tool to study the thermal denaturation of DNA
Authors: Wood, K
Knott, RB
Tonchev, O
Angelov, D
Theodorakopoulos, N
Peyrard, M
Keywords: DNA
Temperature range
Molecules
X-ray equipment
Data
Calorimetry
Issue Date: 2-Oct-2014
Publisher: IOP Science
Citation: Wood, K., Knott, R., Tonchev, O., Angelov, D., Theodorakopoulos, N., & Peyrard, M. (2014). Small-angle scattering as a tool to study the thermal denaturation of DNA. EPL, 108(1), 18002. doi:http://dx.doi.org/10.1209/0295-5075/108/18002
Abstract: DNA thermal denaturation is the breaking of the base pairs, leading to a splitting of the two strands of the double helix. While it is easy to measure the fraction of open base pairs $(f)$ vs. temperature, determining the fraction $(p)$ of fully open molecules is much harder. Previously, the simultaneous recording of f and p could only be achieved for special sequences. We show that small-angle scattering of X-rays or neutrons allows the measurement of p for any sequence. We illustrate the method with a SAXS investigation of two sequences designed to exhibit different melting profiles and compare the SAXS data with nano-calorimetric measurements of the melting curve. © 2014, EPLA.
URI: http://dx.doi.org/10.1209/0295-5075/108/18002
http://apo.ansto.gov.au/dspace/handle/10238/8147
Appears in Collections:Journal Articles

Files in This Item:

There are no files associated with this item.

Items in APO are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback