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Please use this identifier to cite or link to this item: http://apo.ansto.gov.au/dspace/handle/10238/4836

Title: Green Fluorescent Protein Alters the Transcriptional Regulation of Human Mitochondrial Genes After Gamma Irradiation
Authors: Kam, WWY
Middleton, R
Lake, V
Banati, RB
Keywords: DNA
ACTIN
MYOSIN
IONIZATION
RADIATIONS
MUTATIONS
MUSCLES
LIVER
Issue Date: 1-Jul-2013
Publisher: Springer/Plenum Publishers
Citation: Kam, W. W. Y., Middleton, R., Lake, V., & Banati, R. B., (2013). Green Fluorescent Protein Alters the Transcriptional Regulation of Human Mitochondrial Genes After Gamma Irradiation. Journal of Fluorescence, 23 (4), 613-619.
Abstract: Green fluorescent proteins (GFP), extensively used as reporters in biological and imaging studies, are assumed to be mostly biologically inert. Here, we test the assumption in regard to the transcriptional regulation of 18 mitochondrially encoded genes in GFP expressing human T-cell line (JURKAT cells) exposed to gamma radiation. Using quantitative polymerase chain reaction, we demonstrate that wild type and GFP expressing JURKAT cells have different baseline mitochondrial transcript expression (10 out of the 18 tested genes) and after a single dose of radiation (100 Gy) show a significantly different transcriptional regulation of their mitochondrial genes. While in wild type cells, ten of the tested genes are up-regulated in response to radiation exposure, GFP expressing cells show less transcriptional regulation with a small down-regulation in five genes. Our results indicate that the presence of GFP in the cytoplasm can alter the cellular response to ionizing radiation.© 2013, Springer.
URI: http://dx.doi.org/10.1007/s10895-013-1206-x
http://apo.ansto.gov.au/dspace/handle/10238/4836
ISSN: 1053-0509
Appears in Collections:Journal Articles

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