ANSTO Publications Online >
Journal Publications >
Journal Articles >

Please use this identifier to cite or link to this item: http://apo.ansto.gov.au/dspace/handle/10238/3699

Title: The structure of TTHA0988 from Thermus thermophilus, a KipI-KipA homologue incorrectly annotated as an allophanate hydrolase
Authors: Jacques, DA
Langley, DB
Kuramitsu, S
Yokoyama, S
Trewhella, J
Guss, JM
Keywords: Protein
Hydrolases
Bacillus Subtilis
Polypeptides
Genes
Organic Compounds
Issue Date: 1-Feb-2011
Publisher: Wiley-Blackwell
Citation: Jacques, D.A., Langley, D.B., Kuramitsu, S., Yokoyama, S., Trewhella, J., Guss, J.M. (2011). The structure of TTHA0988 from Thermus thermophilus, a KipI-KipA homologue incorrectly annotated as an allophanate hydrolase. Acta Crystallographica Section D-Biological Crystallography, 67(Part 2), 105-111.
Abstract: The Thermus thermophilus protein TTHA0988 is a protein of unknown function which represents a fusion of two proteins found almost ubiquitously across the bacterial kingdom. These two proteins perform a role regulating sporulation in Bacillus subtilis, where they are known as KipI and KipA. kipI and kipA genes are usually found immediately adjacent to each other and are often fused to produce a single polypeptide, as is the case with TTHA0988. Here, three crystal forms are reported of TTHA0988, the first structure to be solved from the family of `KipI-KipA fusion' proteins. Comparison of the three forms reveals structural flexibility which can be described as a hinge motion between the `KipI' and `KipA' components. TTHA0988 is annotated in various databases as a putative allophanate hydrolase. However, no such activity could be identified and genetic analysis across species with known allophanate hydrolases indicates that a misannotation has occurred. © 2011, Wiley-Blackwell. The definitive version is available at www3.interscience.wiley.com
URI: http://dx.doi.org/10.1107/S0907444910051127
http://apo.ansto.gov.au/dspace/handle/10238/3699
ISSN: 0907-4449
Appears in Collections:Journal Articles

Files in This Item:

There are no files associated with this item.

Items in APO are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback