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|Title: ||Detection and quantification of remote microglial activation in rodent models of focal ischaemia using the TSPO radioligand CLINDE.|
|Authors: ||Arlicot, N|
Single Photon Emission Computed Tomography
|Issue Date: ||1-Dec-2010|
|Citation: ||Arlicot, N., Petit, E., Katsifis, A., Toutain, J., Divoux, D., Bodard, S., et al. (2010). Detection and quantification of remote microglial activation in rodent models of focal ischaemia using the TSPO radioligand CLINDE. European Journal of Nuclear Medicine and Molecular Imaging, 37(12), 2371-2380.|
|Abstract: ||Purpose: Neuroinflammation is involved in stroke pathophysiology and might be imaged using radioligands targeting the 18 kDa translocator protein (TSPO). Methods: We studied microglial reaction in brain areas
remote from the primary lesion site in two rodent models of focal cerebral ischaemia (permanent or transient) using [125I]-CLINDE, a promising TSPO single photon emission computed tomography radioligand.
Results: In a mouse model of permanent middle cerebral artery occlusion (MCAO), ex vivo autoradiographic studies demonstrated, besides in the ischaemic territory, accumulation of [125I]-CLINDE in the ipsilateral thalamus with a binding that progressed up to 3 weeks after MCAO. [125I]-
CLINDE binding markedly decreased in animals preinjected with either unlabelled CLINDE or PK11195, while no change was observed with flumazenil pre-treatment, demonstrating TSPO specificity. In rats subjected to transient MCAO, [125I]-CLINDE binding in the ipsilateral thalamus and substantia nigra pars reticulata (SNr) was significantly higher than that in contralateral tissue. Moreover, [125I]-CLINDE binding in the thalamus and SNr was quantitatively correlated to the ischaemic volume assessed
by MRI in the cortex and striatum, respectively. Conclusion: Clinical consequences of secondary neuronal
degeneration in stroke might be better treated thanks to the discrimination of neuronal processes using in vivo molecular
imaging and potent TSPO radioligands like CLINDE to guide therapeutic interventions. © 2010, Springer. The original publication is available at www.springerlink.com|
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