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Please use this identifier to cite or link to this item: http://apo.ansto.gov.au/dspace/handle/10238/2681

Title: Evaluation of radioisotope quality aspects for preparation of high specific activity [Ga-68]-NOTA-AnnexinA1.
Authors: Fuchs, A
Greguric, I
Roe, G
Keywords: Positron Computed Tomography
Radioactivity
High-Performance Liquid Chromatography
Proteins
Gallium 68
Complexometry
Issue Date: 8-Sep-2010
Publisher: Elsevier
Citation: Fuchs, A., Greguric, I., & Roe, G. (2010). Evaluation of radioisotope quality aspects for preparation of high specific activity [Ga-68]-NOTA-AnnexinA1. International Symposium on Technetium and Other Radiometals in Chemistry and Medicine (TERACHEM 2010), 8th - 11th September 2010. Forum Brixen: Bressanone (Bolzano), Italy. In Nuclear Medicine and Biology, 37(6), 692.
Abstract: The bi-functional chelator NOTA-(p-Bn)-NCS permits radio-labelling heat sensitive proteins with Ga-68 (t1/2=68 min) for positron emission tomography. Complexation at room temperature (RT) completes within minutes and in vivo transmetalation is negligible. Here, influences of trace metal cations, on radiochemical yield (RCY) and specific radioactivity (SRA) are assessed. Conjugation of NOTA-(p-Bn)-NCS to AnnexinA1 was performed at varying stoichiometries and conjugates purified by size exclusion high-performance liquid chromatography. Available complexation sites per protein were identified by colorimetric assay and titration with carrier added Ga-67. The complexation of Ga-68 by NOTA-AnnexinA1 at RT and 37°C was systematically studied with and without addition of competing trace metal cations. Ga-67 was used for confirmation of observed trends. Integrity of the radio-conjugate was assessed by addition of up to 104 fold excess of metal cations or apo-transferin and exposure to human serum at 37°C. Gallium-68 gave RCY of N99% within minutes at RT whereas, Ga-67 yielded max 85% dropping as the stock decayed. Presence of Fe(III) showed significant influence on RCY. Once formed, the radio-conjugate showed negligible loss of radioactivity under even the most extreme conditions investigated. SRA and RCY of the radio-conjugate depend significantly on absence of particularly Fe(III) during complexation.
URI: http://dx.doi.org/10.1016/j.nucmedbio.2010.04.148
http://apo.ansto.gov.au/dspace/handle/10238/2681
ISSN: 0969-8051
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