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Title: In vivo deuteration of a native bacterial biopolymer for structural elucidation using SANS.
Authors: Holden, PJ
Russell, RA
Stone, DJM
Garvey, CJ
Foster, LJR
Keywords: Deuteration
Small Angle Scattering
In Vivo
Issue Date: 15-Jul-2004
Publisher: Elsevier
Citation: Holden, P. J., Russell, R. A., Stone, D. J. M., Garvey, C. J., & Foster, L. J. R. (2004). In vivo deuteration of a native bacterial biopolymer for structural elucidation using SANS. Physica B-Condensed Matter, 350(1-3), E643-E646.
Abstract: In order to facilitate future structural studies, biodeuteration of bacterial polyhydroxyalkanoates (PHAs) was investigated. We report here the in vivo deuteration of poly 3-hydroxyoctanoate (PHO) produced by its native host, the bacterium Pseudomonas oleovorans. Bacterial biomass was produced in bioreactor studies by growth on hydrogenated substrates and PHO was subsequently produced intracellularly (10–20% w/w) during batch fed growth on deuterated octanoic acid under oxygen limitation. GC-MS analyses of the PHO demonstrated that 13 of the 15 hydrogen atoms had been replaced with deuterium (except in position 3), the remaining two hydrogen presumably being derived from water. A SANS contrast variation study was conducted on whole cells and the results indicate the potential to discriminate inclusion bodies formed from deuterated precursor from an otherwise hydrogenated background. © 2004, Elsevier Ltd.
ISSN: 0921-4526
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