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|Title: ||Location of sugars in multilamellar membranes at low hydration.|
|Authors: ||Lenne, T|
Small Angle Scattering
|Issue Date: ||15-Nov-2006|
|Citation: ||Lenne, T., Bryant, G., Garvey, C. J., Kelderling, U., & Koster, K. L. (2006). Location of sugars in multilamellar membranes at low hydration. Physica B-Condensed Matter, 385-86, 862-864.|
|Abstract: ||Severe dehydration is lethal for most biological species. However, there are a number of organisms which have evolved mechanisms to avoid damage during dehydration. One of these mechanisms is the accumulation of small solutes (e.g. sugars), which have been shown to preserve membranes by inhibiting deleterious phase changes at low hydration. Specifically, sugars reduce the gel to fluid phase transition temperatures of model lipid/water mixtures. However, there is a debate about the precise mechanism, the resolution of which hinges on the location of the sugars. In excess water, it has been observed using contrast variation SANS that the sugar concentration in the excess phase is higher than in the interlamellar region [Deme and Zemb, J. Appl. Crystallog. 33 (2000) 569]. This raises two questions regarding the location of the sugars at low hydrations: first, does the system phase separate to give a sugar/water phase in equilibrium with a lipid/water/sugar lamellar region (with different sugar concentrations); and second, is the sugar in the interlamellar region uniformly distributed, or does it concentrate preferentially either in close proximity to the lipids, or towards the center of the interbilayer region. In this paper we present the preliminary results of measurements using contrast variation SANS to determine the location of sugars in lipid/water mixtures. © 2006, Elsevier Ltd.|
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