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Title: | Reconstitution of a nanomachine driving the assembly of proteins into bacterial outer membranes |
Authors: | Shen, HH Leyton, DL Shiota, T Belousoff, MJ Noinaj, N Lu, J Holt, SA Tan, K Selkrig, J Webb, CT Buchanan, SK Martin, LL Lithgow, T |
Keywords: | Membranes Pathogens Proteins Molecules Neutron reflectors Pathogens |
Issue Date: | 24-Oct-2014 |
Publisher: | Macmillan Publishers Limited, part of Springer Nature. |
Citation: | Shen, H.-H., Leyton, D. L., Shiota, T., Belousoff, M. J., Noinaj, N., Lu, J., Holt, S. A., Tan, K., Selkrig, J., Webb, C. T., Buchanan, S. K., Martin, L. L., & Lithgow, T. (2014). Reconstitution of a nanomachine driving the assembly of proteins into bacterial outer membranes. Nature Communications, 5. doi:10.1038/ncomms6078 |
Abstract: | In biological membranes, various protein secretion devices function as nanomachines, and measuring the internal movements of their component parts is a major technological challenge. The translocation and assembly module (TAM) is a nanomachine required for virulence of bacterial pathogens. We have reconstituted a membrane containing the TAM onto a gold surface for characterization by quartz crystal microbalance with dissipation (QCM-D) and magnetic contrast neutron reflectrometry (MCNR). The MCNR studies provided structural resolution down to 1 Å, enabling accurate measurement of protein domains projecting from the membrane layer. Here we show that dynamic movements within the TamA component of the TAM are initiated in the presence of a substrate protein, Ag43, and that these movements recapitulate an initial stage in membrane protein assembly. The reconstituted system provides a powerful new means to study molecular movements in biological membranes, and the technology is widely applicable to studying the dynamics of diverse cellular nanomachines. © Macmillan Publishers Limited |
Gov't Doc #: | 7439 |
URI: | http://dx.doi.org/10.1038/ncomms6078 http://apo.ansto.gov.au/dspace/handle/10238/7904 |
Appears in Collections: | Journal Articles |
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