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Title: Cyclization of the Antimicrobial Peptide Gomesin with Native Chemical Ligation: Influences on Stability and Bioactivity
Authors: Chan, LY
Zhang, VM
Huang, YH
Waters, NC
Bansal, PS
Craik, DJ
Daly, NL
Keywords: Peptides
Antimitotic drugs
In vivo
n vitro
Issue Date: 18-Mar-2013
Publisher: Wiley-V C H Verlag
Citation: Chan, L. Y., Zhang, V. M., Huang, Y. H., Waters, N. C., Bansal, P. S., Craik, D. J., & Daly, N. L. (2013). Cyclization of the antimicrobial peptide gomesin with native chemical ligation: influences on stability and bioactivity. ChemBioChem, 14 (5), 617-624. doi:10.1002/cbic.201300034
Abstract: Gomesin is an 18-residue peptide originally isolated from the hemocytes of the Brazilian spider Acanthoscurria gomesiana. A broad spectrum of bioactivities have been attributed to gomesin, including in vivo and in vitro cytotoxicity against tumour cells, antimicrobial, antifungal, anti-Leishmania and antimalarial effects. Given the potential therapeutic applications of gomesin, it was of interest to determine if an engineered version with a cyclic backbone has improved stability and bioactivity. Cyclization has been shown to confer enhanced stability and activity to a range of bioactive peptides and, in the case of a cone snail venom peptide, confer oral activity in a pain model. The current study demonstrates that cyclization improves the in vitro stability of gomesin over a 24 hour time period and enhances cytotoxicity against a cancer cell line without being toxic to a noncancerous cell line. In addition, antimalarial activity is enhanced upon cyclization. These findings provide additional insight into the influences of backbone cyclization on the therapeutic potential of peptides. © 2013, Wiley-VCH Verlag.
Gov't Doc #: 5157
ISSN: 1439-4227
Appears in Collections:Journal Articles

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